PMID: 9175608Mar 1, 1997Paper

Ontogenetic and pharmacological studies on metabotropic glutamate receptors coupled to phospholipase D activation

Neuropharmacology
Jochen KleinK Löffelholz

Abstract

The present study was aimed at characterizing the metabotropic receptor subtype which is involved in the activation of phospholipase D (PLD) by glutamate in rat hippocampal slices. We first observed that the ontogenetic profile of glutamate-induced hydrolysis of phosphoinositides and of phosphatidylcholine was strikingly similar. Both pathways were significantly activated by glutamate in tissue taken from 3-, 8- and 15-day old rats, but not in adult rats. PLD activation was strongest in slices taken from 8-day old rats. At this age, quisqualate had a higher potency for PLD activation (EC50: 0.6 microM) than 1S,3R-ACPD (EC50: 16 microM) and DHPG, a specific activator of group I mGluR, was a full agonist at PLD activation (EC50: 3.5 microM) indicating an involvement of a group I mGluR (mGluR1 and 5). MCPG and AIDA, two putative antagonists at mGluR1 receptors, caused a small but (in the case of MCPG) significant inhibition. DCG-IV, an activator of group II mGluR, was a weak partial agonist at PLD activation (EC50: 22 nM) while L-AP 4, an activator at group III mGluR, was totally inactive. Likewise, forskolin, a stimulant of cyclic AMP formation, was inactive either alone, or in combination with glutamatergic agonists. Pretreatmen...Continue Reading

References

Jan 1, 1992·Journal of Neurochemistry·S K FisherB W Agranoff
Jan 1, 1990·Brain Research. Developmental Brain Research·E PalmerC W Cotman
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F NicolettiE Costa
Oct 1, 1995·Journal of Neurochemistry·J KleinK Löffelholz
Jan 1, 1995·Neuropharmacology·J P Pin, R Duvoisin
May 1, 1994·Neurochemistry International·D D Schoepp
Apr 28, 1995·Journal of Medicinal Chemistry·T KnöpfelH Allgeier
Oct 1, 1993·Journal of Neurochemistry·T HollerK Löffelholz
Apr 14, 1994·Biochimica Et Biophysica Acta·J H Exton
Jun 1, 1996·British Journal of Pharmacology·D E Pellegrini-GiampietroF Moroni

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Citations

Oct 26, 1999·Neuropharmacology·D D SchoeppJ A Monn
Sep 5, 1998·Brain Research. Developmental Brain Research·D ZhaoJ K Blusztajn
Sep 5, 1998·Brain Research. Developmental Brain Research·M A WilsonM V Johnston
Apr 13, 2000·The European Journal of Neuroscience·L PastorinoM Di Luca
Oct 22, 1998·Journal of Cardiovascular Pharmacology·S MaioneF Rossi
Feb 4, 2016·Nature Communications·Kathleen VincentTerence J Coderre
Jul 27, 2005·Journal of Neurochemistry·Jochen Klein
Jan 22, 2005·Journal of Neurochemistry·Jae-Kwang JinYong-Sun Kim
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Mar 18, 2011·The Biochemical Journal·Zoë BarclayRory Mitchell
Jul 12, 2012·Advances in Biological Regulation·Yasukazu Hozumi, Kaoru Goto
Sep 12, 2002·Expert Opinion on Therapeutic Targets·Volker Neugebauer, Susan M Carlton
Oct 3, 1998·The European Journal of Neuroscience·U StrasserD W Choi

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