Ontogenetic and tissue-specific expression of preproghrelin in the Atlantic halibut, Hippoglossus hippoglossus L

The Journal of Endocrinology
Anthony J ManningSusan E Douglas

Abstract

Ghrelin is a conserved vertebrate hormone that affects both GH release and appetite. We have cloned and characterized Atlantic halibut preproghrelin cDNA and examined for the first time preproghrelin expression during fish larval development using quantitative real-time PCR. In addition, cellular sites of expression in larvae and tissue-specific expression in 3-year-old halibut were studied. A full-length cDNA for preproghrelin was isolated from halibut stomach tissue. The 899 bp cDNA encodes an open reading frame of 105 amino acids that is comprised of a signal peptide and two peptides with high similarity to ghrelin and obestatin. The deduced amino acid sequence of halibut ghrelin peptide (GSSFLSPSHKPPKGKPPRA) shows significant conservation relative to other teleostean sequences and is identical to human ghrelin for the first seven amino acids of the sequence. The putative obestatin peptide is well-conserved among fishes but shares limited similarity with its human counterpart. Expression of ghrelin was localized to two different cell types in the stomach of larval halibut by in situ hybridization. However, sensitive PCR assays on tissues collected from 3-year-old fish additionally identified ghrelin transcripts in pyloric ca...Continue Reading

Citations

Jan 17, 2016·General and Comparative Endocrinology·Juan Ignacio BertucciSuraj Unniappan
Apr 6, 2013·General and Comparative Endocrinology·Elisabeth Jönsson
May 10, 2012·General and Comparative Endocrinology·Atsushi SudaHironori Ando
Feb 22, 2012·General and Comparative Endocrinology·Erin ShepperdSuraj Unniappan
Apr 2, 2011·Molecular and Cellular Endocrinology·Andrea RepaciRenato Pasquali
Dec 17, 2009·General and Comparative Endocrinology·Suraj Unniappan
Aug 26, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brian C SmallHiroyuki Kaiya
Dec 17, 2008·General and Comparative Endocrinology·Koji MurashitaIvar Rønnestad
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Nov 5, 2008·Nature Clinical Practice. Endocrinology & Metabolism·Manuel Tena-Sempere
May 2, 2017·Frontiers in Endocrinology·Ivar RønnestadHélène Volkoff

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