PMID: 9192282Mar 1, 1997Paper

Ontogenetic development of diffusional restriction to protein at the pial surface of the rat brain: an electron microscopical study

Journal of Neurocytology
Y BalslevK Møllgård

Abstract

Blood-brain, blood-CSF and ventricular CSF-brain barriers to protein are present very early in brain development. In order to determine whether the outer pial surface of the brain also restricts free penetration of macromolecules, the dorso-lateral part of the sensorimotor cortex from rats at embryonic day 12 (E12), 14, 16, and 18, the day of birth (P0), and adult rat, was studied by electron microscopical techniques. Potassium ferrocyanide, Ruthenium Red and immunogold labelling of endogenous albumin were used to investigate junctional structures and the sites of restriction to albumin diffusion. At E12, large fenestrated sinusoids were present in the pia-arachnoid and the brain surface was formed by an incomplete layer of neuroepithelial and presumptive radial glial end feet, but capillaries in the pia-arachnoid showed no fenestrations at E14 or later. From E14, we observed the progressive appearance of distinct junctional structures between the glial end feet which, to our knowledge, have not been described before. Analysis of albumin distribution from E16 to P0 suggests that the junctions may contribute to restriction of diffusion between the subarachnoid space and the brain extracellular fluid. The restriction to the penet...Continue Reading

Citations

Apr 29, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P D KitchenerN R Saunders
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Eain M Cornford, Shigeyo Hyman
May 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yuhong SuHideaki Tanaka
Mar 13, 2018·The Journal of Physiology·Carina MallardZinaida S Vexler
Apr 30, 2003·The Journal of Comparative Neurology·Carl Joakim EkNorman Ruthven Saunders
Sep 14, 2007·Journal of Anatomy·J P FraherD O'Leary
Jul 16, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Minde I Willardsen, Brian A Link
May 19, 2018·The Journal of Physiology·Norman R SaundersMark D Habgood
Dec 12, 2002·Biological Reviews of the Cambridge Philosophical Society·Michael K Richardson, Gerhard Keuck
Apr 9, 2015·Frontiers in Neuroscience·Christian B BrøchnerKjeld Møllgård
Mar 5, 1999·Clinical and Experimental Pharmacology & Physiology·N R SaundersK M Dziegielewska
Oct 18, 2020·Pharmaceutics·Mark A BryniarskiMarilyn E Morris
May 6, 2021·Physiological Reviews·Martin Kaag RasmussenMaiken Nedergaard
May 16, 2021·Acta Physiologica·Aditya RayasamZinaida S Vexler

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