Ontogeny and characterization of mesenchyme antigens of the sea urchin embryo.

Developmental Biology
C R Tamboline, R D Burke

Abstract

A monoclonal antibody, Sp12, binds to cortical granules, the hyaline layer, and skeletogenic, chromogenic, and blastocoelar mesenchyme of sea urchin eggs and embryos. Adult urchins also express Sp12 antigens in the dermal layer of the test and spines. Antigen is expressed on the surface of primary mesenchyme cells after they have entered the blastocoel, and by two secondary mesenchyme derivatives--the blastocoelar cells after they have been released from the tip of the archenteron, and the pigment cells in prism stage embryos. Immunogold localizations show antigen on the surfaces of mesenchyme, within membrane bounded vesicles, and associated with the Golgi apparatus. Western blots of antigens immunoprecipitated from seven developmental stages reveal twelve antigens ranging in Mr from 35 k to 240 k. Most of these antigens appear, disappear or change Mr over the first five days of development. Characterizations of this complex array of antigens show that the epitope recognized by Sp12 is eliminated by proteolytic enzymes and endoglycosidase F, while immunoreactivity is only reduced by periodate oxidation. As well, calcium magnesium free seawater extracts a subset of antigens different from that retained by crude membrane prepara...Continue Reading

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Citations

Aug 1, 1991·Developmental Biology·R D BurkeC Jackson
Apr 10, 2002·Development, Growth & Differentiation·Tetsuya Kominami, Hiromi Takata
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·Q LiG Stamatoyannopoulos
May 20, 2003·Development, Growth & Differentiation·Tetsuya Kominami, Hiromi Takata
Nov 10, 1998·Developmental Biology·M Marsden, R D Burke
Apr 15, 1992·The Journal of Experimental Zoology·C R Tamboline, R D Burke
Sep 18, 2001·European Journal of Biochemistry·T J Fan, C Katagiri
Aug 26, 2020·The Journal of Comparative Neurology·Laurent FormeryJenifer C Croce

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