Abstract
One of the major beta-cell autoantigens associated with IDDM is GAD. Although GAD expression has been detected in adult islets, transcriptional expression of the GAD genes has not been reported during human pancreatic ontogeny. We therefore analyzed patterns of GAD gene transcription by quantitating the mRNAs encoding both the 65- and 67-kDa isoforms (GAD65 and GAD67, respectively) in human fetal, postnatal, and adult pancreases, as well as in isolated adult islets, and examined their tissue-specific expression. Significant levels of pancreatic GAD65 transcripts were already detected at 13 weeks of gestation and were expressed at higher levels in the fetal and infantile pancreas than in the adult pancreas. Isolated adult pancreatic islets were highly enriched in GAD65 mRNA. In contrast, GAD67 transcripts were not detectable in fetal and postnatal pancreases. In addition to the pancreas, marked GAD expression was detected in the brain, whereas other tissues examined contained either low or undetectable GAD transcripts. Triple immunofluorescent staining of fetal and adult pancreases revealed colocalization of GAD65 with alpha- and beta-cells. In the fetal pancreas, strong immunoreactivity for GAD65 was also evident in epithelial ...Continue Reading
Citations
Jan 23, 1998·Human Immunology·R RajuC S David
Jun 1, 2006·Diabetes Technology & Therapeutics·Megan A WaldropSteven D Chessler
Jan 12, 1999·British Journal of Obstetrics and Gynaecology·J HuG Gennser
May 8, 2003·Canadian Journal of Physiology and Pharmacology·Karima Et-TaouilGérard E Plante
Jul 27, 2007·Endocrine Pathology·Esther KorpershoekRonald R de Krijger
Oct 16, 1999·Brain Research. Molecular Brain Research·D J StoneF M Benes
Apr 27, 2016·Current Diabetes Reports·Vito Lampasona, Daniela Liberati
Aug 19, 2000·American Journal of Physiology. Endocrinology and Metabolism·Y ShiS Baekkeskov
Aug 28, 2002·Diabetes·Kyung Soo KimJi-Won Yoon
Sep 19, 2007·Endocrinology·Simon A Hinke
Jan 21, 2010·Diabetes·Francesco VendrameAlberto Pugliese
Apr 4, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F Homo-Delarche
May 29, 2002·Diabetes·Steven D ChesslerLisa P Hammerle
Feb 15, 2000·The Journal of Biological Chemistry·S D Chessler, A Lernmark
Dec 20, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Adrian VillalbaMarta Vives-Pi
Jun 17, 2015·Scientific Reports·Gucci Jijuan Gu UrbanMasood Kamali-Moghaddam
Sep 7, 2018·Current Diabetes Reports·Fatimah T AlRashidi, Kathleen M Gillespie
Apr 22, 2004·Endocrine Research·Yanlei HaoLei Zhang
Aug 2, 2017·The Journal of Clinical Investigation·Alberto Pugliese
Oct 9, 2002·Journal of Autoimmunity·Sung-E ChoiYup Kang
Sep 7, 2001·Journal of Medicinal Chemistry·K BussE Leistner
Oct 7, 2021·Diabetic Medicine : a Journal of the British Diabetic Association·Anna E LongClaire L Williams