Abstract
The development of bombesin/gastrin-releasing peptide (BN/GRP) binding sites was determined in the rat brain. Using rat brain homogenate, the density of radio-labeled peptide binding sites increased dramatically around birth, remained constant during the first post-natal week, and then increased again between Postnatal (P) Days 7 and 10; the density of binding sites was similar in P10 and adult animals. Rat brain homogenates derived from P1, P7, or P10 animals bound ((125)I-Tyr(4))BN with high affinity (K(d) = 2-4 nM) to a single class of sites (B(max) = 11, 17, and 51 pmol/mg protein, respectively). BN, GRP, and GRP(18-27) inhibited specific radiolabeled peptide binding to rat brain homogenate with high affinity using P1, P3, P7, or P14 animals, whereas GRP(1-16) was inactive. These data indicate that the C-terminal of BN or GRP is essential for high-affinity binding activity in the rat brain during development. In vitro autoradiography revealed high ((125)I-Tyr(4))BN grain densities in the anterior olfactory nucleus, olfactory tubercle, nucleus accumbens, central medial thalamic nucleus, and hippocampus. Moderate grain densities were observed in the parietal cortex, periventricular hypothalamic nucleus, central gray, inferior...Continue Reading
References
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T W MoodyM R Brown
Dec 15, 1977·Life Sciences·M R BrownW W Vale
May 27, 1977·Science·M BrownW Vale
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J F BatteyR I Feldman
Dec 1, 1990·Molecular Endocrinology·E R SpindelT P Segerson
Mar 1, 1991·Neuron·E WadaJ Battery
Dec 24, 1990·Brain Research·E E LadenheimT H Moran
Jul 15, 1985·Life Sciences·Y Tache, M Gunion
Jul 15, 1985·Life Sciences·A CowanD E Gmerek
Jan 1, 1989·Journal of Neural Transplantation·T W MoodyJ J Bernstein
May 1, 1989·Peptides·H C Jackson, I Kitchen
Aug 18, 1987·Neuroscience Letters·R L GetzJ M Rosenstein
Nov 1, 1988·Peptides·V N Hari Prasad, T W Moody
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Sep 1, 1986·The Journal of Comparative Neurology·G M SmithJ Silver
Sep 1, 1986·Peptides·T W MoodyT L O'Donohue
Jan 1, 1985·Peptides·M NambaS R Bloom
Aug 1, 1984·Brain Research·M T Gillati, T W Moody
Mar 1, 1981·The American Journal of Anatomy·R A Rowan, D S Maxwell
Jan 22, 1982·Neuroscience Letters·G P McGregorS R Bloom
May 1, 1982·Peptides·T W MoodyR T Jensen
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E R SpindelJ F Habener
Nov 5, 1984·Life Sciences·A T YachnisT W Moody
Jul 7, 1980·Brain Research·A PertJ Rivier
Nov 1, 1981·Brain Research·J L KentM Herkenham
Apr 1, 1983·Brain Research·J R UnnerstallJ M Palacios
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Rozengurt, J Sinnett-Smith
Jul 1, 1984·Experimental Cell Research·J C WilleyC C Harris
Nov 14, 1984·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Sep 1, 1983·Peptides·Z MeraliS Zalcman
Nov 18, 1982·Brain Research·K A RothJ D Barchas
May 19, 1980·Brain Research·C E Johanson
Jun 1, 1982·Brain Research Bulletin·J A Stuckey, J Gibbs
Oct 1, 1982·Regulatory Peptides·P PanulaE Costa
Jan 1, 1980·Neuropharmacology·R Katz
Jan 1, 1981·Peptides·T W MoodyD M Jacobowitz
May 19, 1980·Life Sciences·T W MoodyC B Pert
Oct 1, 1990·Molecular and Cellular Neurosciences·M C LeeT W Moody