Ontogeny of CX3CR1-EGFP expressing cells unveil microglia as an integral component of the postnatal subventricular zone

Frontiers in Cellular Neuroscience
Anna L XavierJoão R L Menezes

Abstract

The full spectrum of cellular interactions within CNS neurogenic niches is still poorly understood. Only recently has the monocyte counterpart of the nervous system, the microglial cells, been described as an integral cellular component of neurogenic niches. The present study sought to characterize the microglia population in the early postnatal subventricular zone (SVZ), the major site of postnatal neurogenesis, as well as in its anterior extension, the rostral migratory stream (RMS), a pathway for neuroblasts during their transit toward the olfactory bulb (OB) layers. Here we show that microglia within the SVZ/RMS pathway are not revealed by phenotypic markers that characterize microglia in other regions. Analysis of the transgenic mice strain that has one locus of the constitutively expressed fractalkine CX3CR1 receptor replaced by the gene encoding the enhanced green fluorescent protein (EGFP) circumvented the antigenic plasticity of the microglia, thus allowing us to depict microglia within the SVZ/RMS pathway during early development. Notably, microglia within the early SVZ/RMS are not proliferative and display a protracted development, retaining a more immature morphology than their counterparts outside germinal layers. ...Continue Reading

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Citations

Oct 7, 2015·Frontiers in Cellular Neuroscience·Caroline RouauxMarcos R Costa
Sep 7, 2016·Brain, Behavior, and Immunity·Wyston C PierreGregory A Lodygensky
Aug 25, 2016·Experimental Biology and Medicine·Chih Kong Tong, Sharmili Vidyadaran
Aug 9, 2018·Annual Review of Cell and Developmental Biology·Timothy R HammondBeth Stevens
Aug 3, 2019·The EMBO Journal·Vassilis StratouliasBertrand Joseph
Oct 8, 2018·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Hiroyuki KonishiMasaki Ueno
Jan 6, 2021·Biomolecules·Marc MesnilDenis Sarrouilhe
Feb 16, 2021·Frontiers in Oncology·Noelia Geribaldi-DoldánElkin Navarro Quiroz
Jul 6, 2021·RSC Medicinal Chemistry·Silke Miller, Maria-Jesus Blanco
Sep 20, 2021·Anatomical Science International·Yuki Hattori

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Methods Mentioned

BETA
Confocal microscopy
transgenic

Software Mentioned

FIJI
Prism
ImageJ
GraphPad

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