PMID: 7541956Jun 1, 1995Paper

Ontogeny of NO synthase and renin in juxtaglomerular apparatus of rat kidneys

The American Journal of Physiology
E FischerS Bachmann

Abstract

The presence of NO synthase (NOS) in cells of the macula densa (MD) suggests a role for arginine-derived NO in tubulovascular information transfer. To investigate the postnatal development of the neuronal isoform of NOS and of renin in the kidney, the cellular distribution of these enzymes was examined in perfusion-fixed kidneys of 2-, 6-, and 15-day-old rats at both the protein and mRNA level (n = 4 rats/group). NOS and renin and their mRNAs were localized by immunohistochemical and in situ hybridization methods. In addition, NOS levels were assessed by using NADPH diaphorase (NADPH-d) histochemistry. For quantification, the fraction of NOS- and renin-positive glomeruli as well as the number of NOS-positive MD cells was evaluated at all stages. Presence of NOS in single cells of the developing distal tubule was encountered already in the S-shaped body. Full expression of a NOS signal in MD cells was seen as soon as a glomerular urinary space was developed. Double labeling with NADPH-d and antibody to Tamm-Horsfall protein (THP) indicated mutual exclusiveness of NADPH-d-positive MD cells and neighboring THP-positive distal tubule cells at all levels of development. The relative intensity of renin status was 2 day > 6 day > 15 d...Continue Reading

Citations

Jun 10, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M J SolhaugK W Dong
Apr 11, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M J SolhaugK W Dong
Jul 31, 2004·Acta Physiologica Scandinavica·B L JensenO Skøtt
Jun 6, 2012·Pflügers Archiv : European journal of physiology·Jurgen Schnermann, Josephine P Briggs
Dec 2, 2004·American Journal of Physiology. Renal Physiology·Ki-Hwan HanJin Kim
Feb 1, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jane StubbeOle Skøtt
Dec 7, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Ellen Steward PentzR Ariel Gomez
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alp Sener, Francine G Smith
Mar 6, 2009·American Journal of Physiology. Renal Physiology·Björn NeubauerCharlotte Wagner
Sep 18, 2003·American Journal of Physiology. Renal Physiology·Kirsten MadsenBoye L Jensen
Apr 21, 2007·Acta Physiologica Hungarica·L RosivallS MirzaHosseini
Aug 20, 2003·Development·Shigeyasu NakaiTetsuo Noda
Apr 6, 2021·The Journal of Veterinary Medical Science·Akira YabukiOsamu Yamato

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