Ontogeny of the rat hypothalamic nitric oxide synthase and colocalization with neuropeptides

Molecular and Cellular Neurosciences
G TorresC Rivier

Abstract

The topographic ontogeny of nitric oxide synthase (NOS) within the paraventricular nucleus (PVN) of the rat hypothalamus was studied by nicotinamide adenine dinucleotide-diaphorase (NADPH-diaphorase) histochemistry. At Day 1 of postnatal life (P1), NOS-positive neurons were already present and achieved their maturity (in terms of perikarya number and dendritic arborization) about the time of weaning (P21). Across all ages studied (P1 to adulthood), intense NADPH-diaphorase staining was primarily confined within magnocellular cells of the PVN largely characterized by medium-sized (12-15 mum in diameter), ovoid bipolar neurons with prominent clear nuclei. To identify the neurosecretory cells of the adult PVN in which NOS was present, double-labeling studies were carried out via fluorescent immunocytochemistry. Magnocellular oxytocin (OT) and arginine vasopressin (AVP), as well as parvocellular corticotropin-releasing factor (CRF), were found to be colocalized with NOS. However, colocalization occurred significantly more frequently in OT-containing neurons, relative to AVP- or CRF-positive cells. Most of the colocalization occurring between NOS and OT was observed in the rostral constituent of the magnocellular subdivision of the ...Continue Reading

Citations

Apr 3, 2003·The European Journal of Neuroscience·Maria Rosaria MelisAntonio Argiolas
Jun 10, 2003·Annals of the New York Academy of Sciences·Catherine Rivier
Jun 18, 1998·Annals of the New York Academy of Sciences·I Kostoglou-AthanassiouA B Grossman
Mar 1, 2001·Stress : the International Journal on the Biology of Stress·C Rivier
Jan 21, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Shucai LingMarong Fang
Oct 4, 2000·Physiological Reviews·M E FreemanG Nagy
Mar 3, 2015·Physiology·Silvia Graciela RuginskJose Antunes-Rodrigues
Jun 26, 2003·Alcoholism, Clinical and Experimental Research·Soon LeeCatherine Rivier
Apr 29, 1998·European Journal of Pharmacology·A Argiolas, M R Melis
Jul 17, 1999·Brain Research. Brain Research Reviews·T L Krukoff
Dec 17, 1993·Brain Research. Developmental Brain Research·R Wetts, J E Vaughn

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