PMID: 11912059Mar 26, 2002Paper

Oocyte maturation-inducing steroids in protandrous black porgy, Acanthopagrus schlegeli

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Wen-Shiun Yueh, Ching-Fong Chang

Abstract

The study objectives aimed to investigate the maturation-inducing steroid (MIS) in marine protandrous black porgy, Acanthopagrus schlegeli. The characteristics of oocyte maturation were also described. Females were injected with two successive doses of LHRH analog (LHRH-A, 10 and 50 microg/kg of fish). The ovarian tissue was obtained at 6-h intervals for in vitro oocyte maturation. Both 17,20 beta-dihydroxy-4-pregnen-3-one (DHP) and 17,20 beta,21-trihydorxy-4-pregnen-3-one (20 beta-S) were the most effective steroids to induce in vitro maturation (e.g. germinal vesicle breakdown, GVBD) in oocytes cultured for either 24 h or 1 min. 20 beta-S had a better potency than DHP in inducing oocyte maturation. 17-hydroxyprogesterone, 11-deoxycortisol, and 20 beta-21-dihydroxy-4-pregnen-3-one also significantly induced oocyte maturation at high concentrations. The process of oocyte maturation (after the injection of LHRH analog) was founded to be divided into four stages: hormone-insensitive stage (insensitive to gonadotropin and MIS); MIS-insensitive (respond to gonadotropin, but not MIS); MIS-sensitive (respond to MIS); and spontaneous stage (GVBD in the hormone-free condition), respectively. Cycloheximide blocked GVBD at the MIS-insens...Continue Reading

References

Sep 1, 1989·General and Comparative Endocrinology·J M Trant, P Thomas
Sep 1, 1989·General and Comparative Endocrinology·J M Trant, P Thomas
Jul 1, 1993·Fish Physiology and Biochemistry·Y NagahamaM Tanaka

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