Oocyte retrieval after heterotopic transplantation of ovarian tissue cryopreserved by closed vitrification protocol

Journal of Assisted Reproduction and Genetics
Yodo SugishitaNao Suzuki

Abstract

A device for closed vitrification was designed to reduce the risk of contamination and investigated on its efficacy for ovarian function recovery after cryopreservation and heterotopic transplantation. Ovarian tissues from green fluorescence protein transgenic mice (10 GFP mice) were vitrified using the device, and warmed ovarian tissues were transplanted into the ovarian bursa region in wild-type female mice (6 mice). Fresh ovarian tissues were similarly transplanted as a control. After recovery of the estrous cycle, mice were mated with male mice. Ovarian tissues from six cynomolgus monkeys were vitrified and warmed with the device for autologous, heterotopic transplantation. Fresh tissue transplantation was not performed for the control. Ovarian function was examined by recovery of the hormonal cycle. Histological examination was conducted. The number of live pups per recipient mouse was not significantly different after transplantation of fresh or vitrified-warmed ovarian tissue, although the pregnancy rate was reduced with vitrified tissues. The hormonal cycle was restored in 5/6 monkeys after heterotopic transplantation of vitrified-warmed ovarian tissue. Follicles were harvested at eight sites in the omentum and 13 sites...Continue Reading

References

Dec 18, 1992·Science·P MazurA P Mahowald
Aug 1, 1984·Cryobiology·G M FahyH T Meryman
Jul 1, 1994·Journal of Reproduction and Fertility·T SankaiY Yoshikawa
Jul 6, 2000·The New England Journal of Medicine·K Oktay, G Karlikaya
Feb 27, 2003·Biology of Reproduction·Fujio MigishimaMinesuke Yokoyama
Jun 24, 2006·Reproductive Biomedicine Online·Gábor Vajta, Zsolt Péter Nagy
Apr 11, 2009·Human Reproduction·Victoria KerosOuti Hovatta
Apr 30, 2009·Reproductive Biomedicine Online·Noriko KagawaMasashige Kuwayama
Sep 8, 2010·Reproductive Biomedicine Online·Shu HashimotoYoshiharu Morimoto
Jun 17, 2011·Reproductive Biomedicine Online·Christiani A AmorimJacques Donnez
Aug 14, 2012·Fertility and Sterility·Christiani Andrade AmorimAnne Van Langendonckt
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro KawamuraAaron J Hsueh
Oct 27, 2017·The New England Journal of Medicine·Jacques Donnez, Marie-Madeleine Dolmans
Mar 3, 2018·Journal of Assisted Reproduction and Genetics·M G Da BroiP A Navarro

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Citations

Aug 17, 2021·Journal of Assisted Reproduction and Genetics·Yodo SugishitaKutluk Oktay
Sep 30, 2021·Zygote : the Biology of Gametes and Early Embryos·Bárbara Rodrigues NascimentoPaulo Henrique Almeida Campos-Junior

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