Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo development of SCNT embryos in cattle.

PloS One
Jian-Min SuYong Zhang

Abstract

The selection of good quality oocytes is crucial for in vitro fertilization and somatic cloning. Brilliant cresyl blue (BCB) staining has been used for selection of oocytes from several mammalian species. However, the effects of differential oocyte selection by BCB staining on nuclear reprogramming and in vivo development of SCNT embryos are not well understood. Immature compact cumulus-oocyte complexes (COCs) were divided into control (not exposed to BCB), BCB+ (blue cytoplasm) and BCB- (colorless cytoplasm) groups. We found that BCB+ oocytes yielded a significantly higher somatic cell nuclear transfer (SCNT) blastocyst rate and full term development rate of bovine SCNT embryos than the BCB- and control oocytes. BCB+ embryos (embryos developed from BCB+ oocytes) showed increased acetylation levels of histone H3 at K9 and K18 (AcH3K9, AcH3K18), and methylation levels of histone H3 at K4 (H3K4me2) than BCB- embryos (embryos developed from BCB- oocytes) at the two-cell stage. Furthermore, BCB+ embryos generated more total cells, trophectoderm (TE) cells, and inner cell mass (ICM) cells, and fewer apoptotic cells than BCB- embryos. The expression of SOX2, CDX2, and anti-apoptotic microRNA-21 were up-regulated in the BCB+ blastocys...Continue Reading

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Citations

Jan 9, 2014·BMC Genomics·Jianmin SuYong Zhang
Aug 8, 2014·Environmental Science & Technology·Mingzhe FuSong Hua
Jul 24, 2012·Epigenetics & Chromatin·Richard B Meagher, Kristofer J Müssar
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Jan 15, 2014·Molecular Reproduction and Development·Jianmin SuYong Zhang
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Nov 19, 2015·Asian-Australasian Journal of Animal Sciences·Bo FuJing Bai
Apr 14, 2016·Theriogenology·Huanhuan ChenYong Zhang
Jun 22, 2015·Reproductive Biomedicine Online·Inchul ChoiKeith H S Campbell
Mar 10, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Diego Duarte AlcobaIlma Simoni Brum
May 9, 2018·Stem Cells and Development·Elena Gonzalez-Munoz, Jose B Cibelli
Jan 30, 2015·Journal of Assisted Reproduction and Genetics·Zohreh ZareMaryam Dehghani-Mohammadabadi
Sep 27, 2018·Scientific Reports·Wataru IwasakiMasaya Miyazaki
Jun 7, 2019·Zygote : the Biology of Gametes and Early Embryos·Linying JiaEnqi Liu

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Methods Mentioned

BETA
acetylation
PCR
histone acetylation
PCRs
electrophoresis

Software Mentioned

SPSS
Image
Pro plus
TapMan

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