Abstract
In this study, we describe the features of oogenesis in the endoparasitoid, Pteromalus puparum, as well as the distinct type of programmed cell death of the nurse cells through conventional light and fluorescent markers for apoptosis and immunofluorescent analysis. Oogenesis in this endoparasitoid is divided into five stages, of which stages 1-2, 3-4, and 5 are corresponding to previtellogenic growth, yolk uptake, and the formation of egg envelopes, respectively. From these studies, we demonstrate two critical events, which are vitellogenin absorption and rapid transfer of nurse cell content, resulting in remarkable increase in the volume of oocytes during oogenesis in this endoparasitoid. Vitellogenin absorption initiates in the oocyte of early stage 3, and bulk transfer of nurse cell content into the oocyte occurs at stage 4 of oogenesis in P. puparum, which is mainly characterized with the programmed cell death in the nurse cell complex.
References
Aug 1, 1972·British Journal of Cancer·J F KerrA R Currie
Oct 18, 1996·Cell·E S AlnemriJ Yuan
Dec 11, 1997·Cell·M G Vander HeidenC B Thompson
Jun 2, 1998·Development Genes and Evolution·V CavaliereG Gargiulo
Aug 6, 1998·Insect Biochemistry and Molecular Biology·T W Sappington, A S Raikhel
Aug 26, 1998·Cell and Tissue Research·A M FaustoF Giorgi
Jan 23, 1999·Oncogene·A BergmannH Steller
Jul 13, 1999·Cell Motility and the Cytoskeleton·S M Biliński, M K Jaglarz
Oct 24, 2000·European Journal of Cell Biology·I P NezisL H Margaritis
Feb 27, 2001·Cell Motility and the Cytoskeleton·I P NezisL H Margaritis
Mar 21, 2002·Cell and Tissue Research·Ioannis P NezisLukas H Margaritis
May 16, 2003·Tissue & Cell·Ioannis P NezisLukas H Margaritis
Sep 10, 2004·Developmental Biology·Kimberly McCall
Nov 18, 2004·Cell Motility and the Cytoskeleton·Ioannis P NezisLukas H Margaritis
Sep 28, 2006·Cell and Tissue Research·Athanassios D VelentzasLukas H Margaritis
Mar 21, 2007·Journal of Insect Physiology·Sheng-Zhang DongShusheng Liu
Apr 10, 2008·Insect Molecular Biology·G-Y YeC Hu
Jan 1, 1993·Tissue & Cell·J Klag, S Bilinski
Nov 27, 2008·General and Comparative Endocrinology·Sheng-zhang DongCui Hu