OP16 induces deadly autophagy and apoptosis of cells by inhibiting Akt in esophageal squamous cell carcinoma.

Molecular and Cellular Biochemistry
Guiqin HouZhaoming Lu

Abstract

OP16, a derivative of the novel ent-kaurene diterpenoid compound separated from Rabdosia rubescens, has been confirmed for its efficacy and safety in the treatment of esophageal squamous cell carcinoma (ESCC) in our previous study. However, the precise mechanisms of tumor lethality mediated by OP16 have not yet been fully characterized. To investigate the effects and molecular mechanism of OP16 on autophagy and apoptosis of ESCC cells. Effects and mechanism of OP16 on autophagy of ESCC cells were first detected by Western blot, immunofluorescence, mRFP-GFP-LC3 adenovirus infection and transmission electron microscope. Next, function of autophagy and apoptosis induced by OP16 on cell death was investigated by flow cytometry and CCK-8 assay. Finally, molecular mechanism of OP16 affecting autophagy and apoptosis of ESCC cells was explored by Western blot. We demonstrated that OP16 could induce autophagy by promoting the formation of autophagosome and autolysosome, and promote autophagic cell death in ESCC. Furthermore, we also found that OP16 could promote cell apoptosis by activating mitochondria apoptosis pathway in ESCC. Finally, we demonstrated that OP16 affecting autophagy and mitochondria apoptosis pathway was mediated by ph...Continue Reading

References

Jan 10, 2003·Nucleic Acids Research·Christian von MeringBerend Snel
Oct 5, 2006·Cancer Research·Melanie M HippertAndrew Thorburn
Oct 26, 2007·Acta Pharmacologica Sinica·Hong-zhen HuHai-tao Zeng
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Aug 6, 2009·Annual Review of Genetics·Congcong He, Daniel J Klionsky
Aug 27, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eileen White, Robert S DiPaola
Oct 14, 2009·Current Opinion in Cell Biology·Dirk Brenner, Tak W Mak
Mar 17, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jie LiHiroyuki Kuwano
Jan 25, 2011·Nature Cell Biology·Joungmok KimKun-Liang Guan
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jul 23, 2011·Chinese Medicine·Wen TanYitao Wang
Sep 1, 2011·Molecular Cancer Therapeutics·Zhineng J YangFrank A Sinicrope
Feb 15, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
Jan 25, 2014·Cancer Research·David A Gewirtz
Apr 1, 2014·Current Opinion in Cell Biology·Liang GeJames H Hurley
May 31, 2014·Cancer Discovery·Gizem Karsli-UzunbasEileen White
Aug 20, 2014·Nature Reviews. Urology·Jason M FarrowChristopher P Evans
Feb 6, 2015·The Journal of Clinical Investigation·Eileen White
Nov 14, 2015·Autophagy·Michael J Munson, Ian G Ganley
May 28, 2016·British Journal of Clinical Pharmacology·Prabhjot S MundiKevin Kalinsky
Jun 28, 2016·European Journal of Medicinal Chemistry·Ye DingJia Zhou
Sep 25, 2016·Genes & Development·Ravi AmaravadiEileen White
Nov 17, 2016·Stem Cell Research & Therapy·Areechun SotthibundhuSanbing Shen
Jan 13, 2017·Journal of Molecular Biology·Fulvio Reggiori, Christian Ungermann
Nov 14, 2017·Autophagy·Alexandra C RacanelliSuzanne M Cloonan
Dec 14, 2017·Essays in Biochemistry·Cansu KarabiyikDavid C Rubinsztein
Apr 21, 2018·Current Opinion in Pharmacology·Timothy M HallKelly A Whelan

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
Z-VAD-FMK

Methods Mentioned

BETA
flow cytometry
transmission electron microscopy

Software Mentioned

SPSS21
GraphPad Prism
ImageJ

Related Concepts

Related Feeds

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis