Open-and-shut cases in coiled-coil assembly: alpha-sheets and alpha-cylinders

Protein Science : a Publication of the Protein Society
John Walshaw, D N Woolfson

Abstract

The coiled coil is a ubiquitous protein-folding motif. It generally is accepted that coiled coils are characterized by sequence patterns known as heptad repeats. Such patterns direct the formation and assembly of amphipathic alpha-helices, the hydrophobic faces of which interface in a specific manner first proposed by Crick and termed "knobs-into-holes packing". We developed software, SOCKET, to recognize this packing in protein structures. As expected, in a trawl of the protein data bank, we found examples of canonical coiled coils with a single contiguous heptad repeat. In addition, we identified structures with multiple, overlapping heptad repeats. This observation extends Crick's original postulate: Multiple, offset heptad repeats help explain assemblies with more than two helices. Indeed, we have found that the sequence offset of the multiple heptad repeats is related to the coiled-coil oligomer state. Here we focus on one particular sequence motif in which two heptad repeats are offset by two residues. This offset sets up two hydrophobic faces separated by approximately 150 degrees -160 degrees around the alpha-helix. In turn, two different combinations of these faces are possible. Either similar or opposite faces can int...Continue Reading

References

Jan 27, 1989·Science·E K O'SheaP S Kim
Jan 30, 1997·Nature·M WienerR M Stroud
Jun 11, 1999·Current Opinion in Structural Biology·M R Groves, D Barford
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne

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Citations

Nov 11, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sara E CnuddeJames H Geiger
Dec 4, 2003·Journal of Structural Biology·John Walshaw, Derek N Woolfson
Oct 18, 2002·Advanced Drug Delivery Reviews·Y Bruce Yu
Nov 1, 2011·Nature Chemical Biology·Nathan R ZaccaiDerek N Woolfson
Nov 7, 2012·Bioinformatics·Thomas L VincentDerek N Woolfson
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jie LiuMin Lu
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Daniela AlbanesiAlejandro Buschiazzo
Apr 9, 2001·Journal of Molecular Biology·J Walshaw, D N Woolfson
Jun 1, 2011·Biomolecular Concepts·Zoltán Gáspári, László Nyitray
Oct 25, 2014·Science·Andrew R ThomsonDerek N Woolfson
Oct 13, 2006·Current Opinion in Chemical Biology·Derek N Woolfson, Maxim G Ryadnov
Jan 27, 2015·Structure·E H EgelmanV P Conticello
Dec 19, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·A MarkeyA Saiani
Dec 14, 2006·Angewandte Chemie·Maxim G Ryadnov
Jul 13, 2013·The Journal of Biological Chemistry·Karsten BrandtGabriele Deckers-Hebestreit
Oct 10, 2018·Nature Communications·Guto G RhysDerek N Woolfson
Jun 27, 2018·ACS Synthetic Biology·Franziska ThomasDerek N Woolfson
Jun 17, 2004·Journal of the American Chemical Society·Maxim G Ryadnov, Derek N Woolfson
Aug 2, 2003·Journal of the American Chemical Society·Maxim G RyadnovDerek N Woolfson

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