Open reading frame 193R of Chilo iridescent virus encodes a functional inhibitor of apoptosis (IAP)

Virology
Ikbal Agah InceMonique M van Oers

Abstract

Programmed cell death or apoptosis is a major defense mechanism in insects in response to viral infections. The genome of Chilo iridescent virus (CIV) has three ORFs with homology to baculovirus inhibitor of apoptosis (iap) genes. The proteins encoded by the 157L, 193R, and 332L ORFs contain 152, 208 and 234 amino acids, respectively. While all three proteins contain C-terminal RING domains, only the protein encoded by ORF 193R contains a baculoviral iap repeat (BIR) domain, indicative of a putative IAP protein. The 193R protein has 28 and 27% similarity in amino acid sequence to the Orgyia pseudotsugata MNPV and Cydia pomonella granulovirus IAP-3 proteins, respectively. ORF 193R from CIV is the only gene known to exist among the Iridoviridae that encodes a BIR domain. 193R is transcribed early during CIV infection, and its transcription is not dependent on the synthesis of early viral proteins. When this putative CIV IAP was transiently expressed in SPC-BM-36 and Sf21 cells under the control of an immediate early baculovirus promoter it significantly reduced apoptosis induced by actinomycin-D. Silencing of the CIV iap gene (193R) in CIV infected SPC-BM-36 cells with 193R-specific dsRNA resulted in apoptosis. Thus, CIV ORF 193R...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E S AlnemriG Litwack
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R Goorha, K G Murti
Aug 1, 1994·Molecular and Cellular Biology·R J Clem, L K Miller
Sep 1, 1996·Protein Expression and Purification·D L JarvisL A Guarino
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 20, 1997·Journal of Cellular Physiology·L K Miller
Feb 17, 1999·Genes & Development·Q L Deveraux, J C Reed
Sep 7, 2001·Virology·H Everett, G McFadden
Sep 29, 2001·Journal of Virological Methods·M ConstantinoT Williams
Dec 26, 2001·Pharmacology & Therapeutics·K C ZimmermannD R Green
Jan 5, 2002·Archives of Virology·S M D'CostaS L Bilimoria
Dec 31, 2003·Virology·Remziye NalçacioğluMonique M van Oers
Aug 17, 2006·Journal of Virology·Gustavo DelhonDaniel L Rock
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Brian LeberDavid W Andrews
Aug 3, 2007·Journal of Biochemistry and Molecular Biology·Yu-Sin KimYi-Peng Qi
Aug 19, 2007·The Journal of General Virology·Remziye NalçacioğluMonique M van Oers
Nov 6, 2007·Current Drug Targets·Monique M van Oers, Just M Vlak
Nov 6, 2007·Current Drug Targets·Rollie J Clem

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Citations

Apr 1, 2014·Journal of Invertebrate Pathology·Amélie SegarraTristan Renault
Jun 17, 2014·Virus Research·Arzu OzgenRemziye Nalcacioglu
Oct 14, 2011·Journal of Virology·Nilesh S ChitnisShan L Bilimoria
Oct 12, 2012·The Journal of General Virology·Ikbal Agah InceMonique M van Oers
Feb 6, 2015·Seminars in Cell & Developmental Biology·Rollie J Clem
Jul 3, 2016·Journal of Invertebrate Pathology·Remziye NalcaciogluZihni Demirbag
Sep 7, 2016·Journal of Invertebrate Pathology·Trevor WilliamsMonique M van Oers
Jan 1, 2013·Cells·Jean Berthelet, Laurence Dubrez
Mar 31, 2018·Viruses·İkbal Agah İnceArzu Özgen
Dec 13, 2018·The Journal of General Virology·Zi-Shu ChenGuo-Hua Huang
Apr 27, 2013·The Journal of General Virology·Srini PereraBasil Arif
Nov 1, 2020·Journal of Invertebrate Pathology·Aydin YesilyurtRemziye Nalcacioglu

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis