Open-tubular capillary electrochromatography using a capillary coated with octadecylamine-capped gold nanoparticles

Electrophoresis
Qishu QuChao Yan

Abstract

Octadecylamine-capped gold nanoparticles (ODA-Au-NPs) were prepared and characterized by using UV-Vis adsorption spectrum, transmission electron chromatography (TEM), SEM, and FT-IR. A simple but robust hydrophobic coating was easily developed by flushing a capillary with a solution of ODA-Au-NPs, because the positive charges were carried by the nanoparticles which strongly adsorb to the negatively charged inner surface of a fused-silica capillary via electrostatic and hydrophobic interactions. The chromatographic characteristics of the coated capillary was investigated by varying the experimental parameters such as buffer pH, buffer concentration, and percentage of organic modifier in the mobile phase. The results show that (i) resolution between thiourea and naphthalene is almost the same when comparing the electrochromatograms obtained using pH 7 buffer as mobile phase after and before the capillary column was operated using pH 11 and 3 mobile phase; (ii) no significant changes in retention time and deterioration in peak efficiency were found after 60 runs of test aromatic mixtures; and (iii) column efficiency up to 189 000 theoretical plates/meter for testosterone was obtained. All of the results indicated that the coating ...Continue Reading

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Citations

Jun 12, 2009·Electrophoresis·Frantisek Svec
Sep 9, 2010·Journal of Separation Science·Xiaowei ZhangZenghong Xie
Nov 30, 2011·Journal of Separation Science·Pavel ŘezankaVáclav Kašička
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Dec 21, 2013·The Analyst·David A CollinsBrett Paull
Jul 14, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova
May 28, 2019·Nature Communications·Kristina R RusimovaVentsislav K Valev
Oct 28, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuanyuan LiuChao Yan

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