Operational Performance of the Onchocerca volvulus "OEPA" Ov16 ELISA Serological Assay in Mapping, Guiding Decisions to Stop Mass Drug Administration, and Posttreatment Surveillance Surveys

The American Journal of Tropical Medicine and Hygiene
Frank O RichardsT R Unnasch

Abstract

Onchocerciasis is a neglected tropical disease targeted for elimination. The World Health Organization (WHO) has developed guidelines for the verification of onchocerciasis elimination that include entomological and epidemiological criteria. The latter require demonstrating with statistical confidence that the infection prevalence in children is less than 0.1%, necessitating an assay with a high degree of specificity. We present an analysis of the performance of the Onchocerciasis Elimination Program for the Americas (OEPA) version of the Ov16 enzyme-linked immunosorbant assay (ELISA) when used under operational conditions. In Africa and Latin America, the assay demonstrated 99.98% specificity in 69,888 children in 20 foci where transmission was believed to be interrupted. The assay produced a prevalence estimate equal to that of skin snip microscopy when applied in putatively hypo-endemic zones of Ethiopia. The OEPA Ov16 ELISA demonstrated the specificity required to be effectively deployed to verify transmission elimination under the WHO guidelines, while exhibiting a sensitivity equivalent to skin snip microscopy to identify hypo-endemic areas.

References

May 14, 2009·PLoS Neglected Tropical Diseases·Peter D BurbeloThomas B Nutman
Jul 3, 2010·The American Journal of Tropical Medicine and Hygiene·Mario A Rodríguez-PérezGibert Prado-Velasco
Sep 13, 2012·Journal of Parasitology Research·Nancy Cruz-OrtizNidia Rizzo
Apr 5, 2013·PLoS Neglected Tropical Diseases·Mario A Rodríguez-PérezFrancisco Gibert Prado-Velasco
May 22, 2013·The American Journal of Tropical Medicine and Hygiene·Tarig B HigaziKamal Hashim
Dec 18, 2013·The American Journal of Tropical Medicine and Hygiene·David OguttuThomas R Unnasch
Feb 7, 2014·Parasites & Vectors·Jacinto ConvitMaría E Grillet
Apr 2, 2014·The American Journal of Tropical Medicine and Hygiene·Moses KatabarwaFrank O Richards
Nov 4, 2016·The American Journal of Tropical Medicine and Hygiene·Isam M A ZarrougTarig B Higazi
Jan 10, 2018·The American Journal of Tropical Medicine and Hygiene·Ole LagatieLieven J Stuyver
Feb 23, 2018·International Health·Thomas R UnnaschPaul T Cantey
Apr 13, 2018·Parasites & Vectors·Rubén Santiago NichollsGloria Inés Palma

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