PMID: 7539919Jun 6, 1995Paper

Operational RNA code for amino acids: species-specific aminoacylation of minihelices switched by a single nucleotide

Proceedings of the National Academy of Sciences of the United States of America
D HippsP Schimmel

Abstract

The genetic code is based on aminoacylation reactions where specific amino acids are attached to tRNAs bearing anticodon trinucleotides. However, the anticodon-independent specific aminoacylation of RNA minihelix substrates by bacterial and yeast tRNA synthetases suggested an operational RNA code for amino acids whereby specific RNA sequences/structures in tRNA acceptor stems correspond to specific amino acids. Because of the possible significance of the operational RNA code for the development of the genetic code, we investigated aminoacylation of synthetic RNA minihelices with a human enzyme to understand the sequences needed for that aminoacylation compared with those needed for a microbial system. We show here that the species-specific aminoacylation of glycine tRNAs is recapitulated by a species-specific aminoacylation of minihelices. Although the mammalian and Escherichia coli minihelices differ at 6 of 12 base pairs, two of the three nucleotides essential for aminoacylation by the E. coli enzyme are conserved in the mammalian minihelix. The two conserved nucleotides were shown to be also important for aminoacylation of the mammalian minihelix by the human enzyme. A simple interchange of the differing nucleotide enabled t...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C P LeeU L RajBhandary
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A Martinis, P Schimmel
Jul 1, 1993·Nucleic Acids Research·S SteinbergM Sprinzl
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·D D Buechter, P Schimmel
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P SchimmelS Yokoyama

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Citations

Mar 29, 2000·Biopolymers·P J Beuning, K Musier-Forsyth
Oct 15, 2009·Journal of Molecular Evolution·Junichi SugaharaAkio Kanai
Sep 24, 1999·Biochimie·S A MartinisC Florentz
Jan 20, 1998·Trends in Biochemical Sciences·K ShibaP Schimmel
Jun 1, 1997·Bioorganic & Medicinal Chemistry·B S Henderson, P Schimmel
Sep 5, 2001·The EMBO Journal·M A LovatoP Schimmel
Nov 4, 1998·Nucleic Acids Research·R GiegéC Florentz
Feb 14, 2006·Nucleic Acids Research·David H Ardell, Siv G E Andersson
Jan 21, 2014·RNA·Hubert H Rogers, Sam Griffiths-Jones
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·R S Lipman, Y M Hou
Aug 1, 2015·Nucleic Acids Research·Abdullah H SahyounMatthias Bernt
Apr 1, 2004·Molecular Cell·Martha A LovatoPaul Schimmel
Dec 10, 1999·The Journal of Biological Chemistry·B A Steer, P Schimmel
Nov 1, 2000·The Journal of Biological Chemistry·J D DignamJ B Chaires
Mar 10, 2001·The Journal of Biological Chemistry·L Ribas de Pouplana, P Schimmel
Jan 19, 2018·RNA Biology·Joanne M HoCorwin A Miller
May 9, 2001·The Journal of Biological Chemistry·B BurkeY M Hou
Aug 3, 2019·Genes·Abdoallah SharafMiroslav Oborník
May 9, 1997·Journal of Molecular Biology·N NamekiT Hasegawa
Feb 5, 2008·Methods : a Companion to Methods in Enzymology·Marie SisslerCatherine Florentz
Nov 11, 2021·Microbiology and Molecular Biology Reviews : MMBR·Madhan R TirumalaiGeorge E Fox

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