Operomics: integrated genomic and proteomic profiling of cells and tissues

Briefings in Functional Genomics & Proteomics
Samir Hanash, Laura M Beretta

Abstract

In the post-genome era, technologies are becoming available that allow the profiling of tissues and cell populations at multiple levels including genomic (DNA and RNA), proteomic (proteins and peptides) and post-proteomic (eg metabolomic). Operomics refers to the molecular analysis of tissues and cells at the three levels that are connected through the coding process - namely, DNA, RNA and protein. The premise is that no one level or type of analysis fully captures gene expression and that functional changes at the proteome level cannot be simply predicted from analyses at the DNA or RNA levels. An important determinant that weakens a direct link between RNA and protein levels is translational control that differentially regulates mRNA translation. In this paper, the approaches for genomic and proteomic profiling and the contribution of translational control are reviewed.

Citations

Jun 13, 2012·Journal of Proteome Research·Hyo-Jung ChoiTae-Hwan Kwon
Apr 26, 2013·Journal of Proteome Research·Brian A RiskMorgan C Giddings
Jan 7, 2005·The FEBS Journal·Julio E CelisPavel Gromov
Feb 22, 2012·Archivos de la Sociedad Española de Oftalmología·M Dolores Pinazo-Durán
May 10, 2017·Omics : a Journal of Integrative Biology·Kishore GollapalliSanjeeva Srivastava
Jan 20, 2010·Toxicology Mechanisms and Methods·Seema SinghMahendra Pratap Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.