Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells

Journal of Integrative Medicine
Qing-qing ZangCheng Huang

Abstract

To investigate the effects of ophiopogonin D on human breast cancer MCF-7 cells. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation experiments. Cell cycle was measured with cell cycle flow cytometry and a living cell assay. Apoptosis and terminal deoxynucleoitidyl transferase-mediated dUTP nick end labeling assays were performed to detect the apoptosis of MCF-7 cells induced by ophiopogonin D. Finally, Western blotting was used to explore the mechanism. Exposure of cells to ophiopogonin D resulted in marked decreases in viable cells and colony formation with a dose-dependent manner. Treatment of these cells with ophiopogonin D also resulted in cell cycle arrest at the G(2)/M phase, and increased apoptosis. Mechanistically, ophiopogonin D-induced G(2)/M cell cycle arrest was associated with down-regulation of cyclin B1. Furthermore, activation of caspase-8 and caspase-9 was involved in ophiopogonin D-induced apoptosis. The data suggested that ophiopogonin D inhibits MCF-7 cell growth via the induction of cell cycle arrest at the G(2)/M phase.

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Citations

Nov 11, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Liang PengShunchang Jiao
Jul 1, 2017·Journal of Integrative Medicine·Chun-Song Hu, Tengiz Tkebuchava
Nov 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Siyu ChenXiao Han
Dec 3, 2016·Oncotarget·Chenyang YeYing Chai
May 31, 2018·Molecular Medicine Reports·Gang QuJiahui Yang
Aug 25, 2017·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·X G ChenJ Ma
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Dec 26, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaoqin ZhuMeihong Luo
Aug 3, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Yueyang FanYinglu Feng
Jan 10, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Xin LengJian Ni
Jan 16, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Ziyu ZhangOuping Huang
Aug 21, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xue ZhuYu Chen

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