Opioid modulation of capsaicin-evoked release of substance P from rat spinal cord in vivo.

Peptides
L D Aimone, Tony L Yaksh

Abstract

Capsaicin has been shown to evoke the release of substance P (SP) from small diameter primary afferent fibers. Using an in vivo perfusion of the rat spinal cord, this study examined the pharmacology of opioid receptor systems which modulate the capsaicin-evoked release of SP. The addition of capsaicin (200 microM) to the perfusate raised SP-like immunoreactivity (SP-LI) from resting levels of 31 +/- 5 to 74 +/- 14 pg/ml or an increase of 139% above the baseline. Using high pressure liquid chromatography (HPLC) the identity of the released SP-LI was determined to coelute primarily with authentic SP or the oxidized form of SP. Opioid receptor agonists were added to the perfusate and their ability to inhibit capsaicin-evoked release of SP-LI was assessed. Morphine (10-100 microM), DAGO (1-100 microM), DPLPE (10-100 microM), but not U50488H (100 microM) produced a dose-dependent reduction in the capsaicin-evoked release of SP-LI. Pretreatment with the opioid receptor antagonist naloxone (1 mg/kg, IP) had no effect on the basal or capsaicin-evoked release of SP-LI. Naloxone pretreatment was able to antagonize completely the opioid-produced inhibition of capsaicin-evoked SP-LI release. These data indicate that the release of SP from ...Continue Reading

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Citations

Jan 1, 1995·Pharmacology & Therapeutics·M Satoh, M Minami
May 26, 1998·European Journal of Pharmacology·J D RichardsonK M Hargreaves
Jan 1, 1997·Acta Anaesthesiologica Scandinavica·T L Yaksh
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Jun 16, 2009·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Krishna KumarSherri Tracey
May 11, 2007·Best Practice & Research. Clinical Anaesthesiology·Wolfgang Koppert, Martin Schmelz

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