PMID: 8584761Oct 20, 1995Paper

Opioid peptide receptor studies. 4. Antisense oligodeoxynucleotide to the delta opioid receptor delineates opioid receptor subtypes

Regulatory Peptides
X Y ChaR B Rothman

Abstract

Prior work in our laboratory has identified putative subtypes of delta (delta cx-1, delta cx-2, delta ncx-1, delta ncx-2) and kappa 2 (kappa 2a and kappa 2b) receptors. Previous studies showed that chronic (three day) i.c.v. administration of antisense oligodeoxynucleotide to the cloned delta opioid receptor selectively decreased [3H][D-Ala2,D-Leu5]enkephalin binding to the delta ncx site, not the delta cx-2 site. The present study extends this work by demonstrating that delta antisense DNA selectively affects the delta ncx-2 site sparing the other putative delta receptor subtypes and kappa 2 receptor subtypes. This selectivity is not due to anatomically specific effects of delta antisense DNA since autoradiograms show that delta binding is reduced in all regions of the brain after chronic i.c.v. administration of delta antisense DNA. These data strongly suggest that the delta cx-1, delta cx-2, delta ncx-1, kappa 2a and kappa 2b binding sites are different proteins than the delta ncx-2 binding site, which, based on its sensitivity to delta antisense DNA, is synonymous to the cloned delta opioid receptor. Viewed collectively, these data suggest that administration of delta antisense DNA, and by extension other receptor-selective...Continue Reading

References

Jun 9, 1977·Nature·J A LordH W Kosterlitz
Jun 5, 1992·European Journal of Pharmacology·M SofuogluA E Takemori
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A RichardsonE A Barnard
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B L KiefferC G Hirth
Dec 28, 1992·Science·C J EvansR H Edwards
Sep 1, 1991·Pharmacopsychiatry·T F MeertP A Janssen
Jul 1, 1987·Neuropeptides·J SimonM Wollemann
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W F SimondsW A Klee
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T M ChoH H Loh
Aug 1, 1981·British Journal of Pharmacology·H W KosterlitzL E Robson
Aug 1, 1995·Anesthesia and Analgesia·J G D'Alessio
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J B WangG R Uhl
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K YasudaG I Bell

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