PMID: 8464907Apr 1, 1993Paper

Opioid peptides activate phospholipase D and protein kinase C-epsilon in chicken embryo neuron cultures

Proceedings of the National Academy of Sciences of the United States of America
D Mangoura, G Dawson

Abstract

The mu-opioid peptide morphiceptin stimulated a Ca(2+)-independent protein kinase C (PKC-epsilon) that is expressed both in embryonic day 6 chicken telencephalon and in derived neuronal cultures. This activation was seen as a 2-fold increase in the activity and level of cytosolic PKC-epsilon and as a transient increase in membrane-associated PKC-epsilon following morphiceptin treatment. Morphiceptin did not activate phospholipase C-mediated phosphatidylinositol hydrolysis but did transiently activate (2- to 3-fold) phospholipase D (PLD), as measured by phosphatidylethanol formation in neuron cultures derived from embryonic day 6 or day 7 cerebral hemispheres. This PLD activation could provide an alternative source of diacylglycerol for the activation of PKC-epsilon and was naloxone-reversible and at least partially blocked by the tyrosine kinase inhibitor herbimycin A. Addition of phorbol 12-myristate 13-acetate stimulated both PLD and PKC-epsilon activities to a greater extent than opioids. The phorbol ester and insulin stimulation of PLD was also blocked by herbimycin. Both morphiceptin (in a naloxone-reversible manner) and phorbol ester increased phosphorylation of similar cytosolic proteins in intact cells, demonstrating a ...Continue Reading

References

Mar 16, 1992·Biochemical and Biophysical Research Communications·T IshizukaR V Farese
Nov 1, 1991·Trends in Pharmacological Sciences·N T ThompsonL G Garland
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H KoideY Nishizuka
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L M PfefferB Strulovici
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R H CarterD T Fearon
Jan 1, 1990·Annals of the New York Academy of Sciences·A VernadakisD Mangoura
May 7, 1990·FEBS Letters·R W RandallL G Garland
Jul 31, 1990·European Journal of Biochemistry·D SchaapP J Parker
Oct 1, 1988·Trends in Neurosciences·R J Miller
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N Sakellaridis, A Vernadakis

❮ Previous
Next ❯

Citations

Nov 28, 2000·Cell Motility and the Cytoskeleton·S ChengV Rehder
Feb 15, 1993·Journal of Neuroscience Research·G DawsonG R Post
May 24, 1996·Chemistry and Physics of Lipids·S C Olson, J D Lambeth
May 24, 1996·Chemistry and Physics of Lipids·M Liscovitch, V Chalifa-Caspi
Feb 12, 2000·Progress in Neurobiology·E J HorváthM Palkovits
Jun 11, 1999·Journal of the American College of Cardiology·F TomaiP A Gioffré
Sep 9, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D MangouraD X Wang
Mar 15, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G Dawson
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·C LehelW B Anderson
Jan 12, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jin-Long LiZhao-Xin Tang
Aug 6, 2013·Behavioural Brain Research·Styliani ZisopoulouDimitra Mangoura
Mar 27, 1996·Biochimica Et Biophysica Acta·B Y KimJ S Ahn
May 17, 2018·Metallomics : Integrated Biometal Science·Wei LiJin-Long Li
Feb 18, 2005·Australasian Psychiatry : Bulletin of Royal Australian and New Zealand College of Psychiatrists·Roy KrawitzCris Mileshkin
May 15, 2001·American Journal of Physiology. Cell Physiology·N A BourbonM Kester
Apr 24, 1999·European Journal of Biochemistry·G GaibeletL J Emorine
Dec 6, 1997·Biochemical and Biophysical Research Communications·L G LouG Pei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.