Opioid receptors on guinea-pig intestinal crypt epithelial cells

The Journal of Physiology
M E LangJ B Meddings

Abstract

1. Opioid peptides promote net intestinal absorption via two mechanisms: stimulation of Na+ and Cl- absorption and inhibition of Cl- secretion. Although these transport changes are predominantly mediated by submucosal neurones, it is currently unclear whether opioid peptides can regulate enterocyte function directly. We therefore tested the hypothesis that enterocytes have specific opioid receptors. 2. Villus and crypt jejunal epithelial cells were isolated by the distended sac method from anaesthetized guinea-pigs. Flow cytometry was used to resolve enterocytes from other cell types and to determine whether binding of a fluorescently labelled opioid antagonist, naltrexone-FITC, could be prevented by unlabelled mu- and delta-opioid receptor agonists. A population of crypt enterocytes (approximately 21%) exhibited high-affinity naltrexone-FITC binding to both mu- and delta-type binding sites that was stereoselective and sodium dependent. Villus enterocytes did not exhibit any of these characteristics. 3. Basal cAMP production was elevated in both villus and crypt cells treated with IBMX (3-isobutyl-1-methylxanthine). Villus cells did not respond to 100 nM vasoactive intestinal peptide (VIP), nor were they affected by opioid pept...Continue Reading

Citations

Aug 23, 2011·Progress in Neurobiology·Fadi H Mourad, Nayef E Saadé
May 1, 2007·European Journal of Pharmacology·Carolyn J BagloleJonathan B Meddings
Nov 9, 2004·Life Sciences·Kamyar GhaffariAhmad Reza Dehpour
Mar 18, 2006·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Tajemah MombeiniAhmad Reza Dehpour
Sep 26, 2015·European Journal of Pharmacology·Marta ZielińskaJakub Fichna
Nov 23, 2007·FEMS Immunology and Medical Microbiology·David R Brown, Lisa D Price
Jul 11, 2001·Scandinavian Journal of Gastroenterology·H J EppleJ D Schulzke
Sep 26, 2015·Nutrients·Sebely PalSuleen Ho
Dec 17, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sandra ZoghbiPascale Plaisancié
Feb 9, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Amanda J PageL Ashley Blackshaw
Jul 17, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Fan ChengIan S Zagon
Feb 28, 2002·The Journal of Pharmacology and Experimental Therapeutics·DeWayne Townsend, David R Brown
May 18, 2005·Canadian Journal of Physiology and Pharmacology·Carolyn J BagloleJonathan B Meddings
Aug 30, 2019·Current Pediatric Reviews·Sonal GuptaPraveen Mathur
Oct 2, 1998·The American Journal of Physiology·K A BaradaC F Nassar
Mar 4, 2003·Journal of Applied Physiology·Lian WangDonovan B Yeates
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·D A YatesM H Perdue
Feb 6, 2020·Frontiers in Immunology·Jingjing MengSabita Roy
Oct 7, 2006·Autonomic Neuroscience : Basic & Clinical·David E Burleigh, Matthew R Banks

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.