Opportunistic use of catecholamine neurotransmitters as siderophores to access iron by Pseudomonas aeruginosa

Environmental Microbiology
Quentin PerraudIsabelle J Schalk

Abstract

Iron is an essential nutrient for bacterial growth and the cause of a fierce battle between the pathogen and host during infection. Bacteria have developed several strategies to access iron from the host, the most common being the production of siderophores, small iron-chelating molecules secreted into the bacterial environment. The opportunist pathogen Pseudomonas aeruginosa produces two siderophores, pyoverdine and pyochelin, and is also able to use a wide panoply of xenosiderophores, siderophores produced by other microorganisms. Here, we demonstrate that catecholamine neurotransmitters (dopamine, l-DOPA, epinephrine and norepinephrine) are able to chelate iron and efficiently bring iron into P. aeruginosa cells via TonB-dependent transporters (TBDTs). Bacterial growth assays under strong iron-restricted conditions and with numerous mutants showed that the TBDTs involved are PiuA and PirA. PiuA exhibited more pronounced specificity for dopamine uptake than for norepinephrine, epinephrine and l-DOPA, whereas PirA specificity appeared to be higher for l-DOPA and norepinephrine. Proteomic and qRT-PCR approaches showed pirA transcription and expression to be induced in the presence of all four catecholamines. Finally, the oxidat...Continue Reading

References

Jan 1, 1992·Life Sciences·M Lyte, S Ernst
Dec 1, 1990·Journal of Bacteriology·Keith PooleS Neshat
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Jul 1, 1973·Annals of Surgery·A C GrovesR N Meek
Mar 12, 1970·Biochemical and Biophysical Research Communications·J R Pollack, J B Neilands
Apr 1, 2000·Applied and Environmental Microbiology·A S Cornish, W J Page
Mar 14, 2002·Journal of the American Chemical Society·Martin E BluhmKenneth N Raymond
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kenneth N RaymondSanggoo S Kim
Jun 9, 2004·Microbiology·Bart GhyselsPierre Cornelis
Aug 20, 2004·Pharmacological Reviews·Graeme EisenhoferDavid S Goldstein
Feb 28, 2006·Journal of Molecular Biology·Gaëtan L A MislinIsabelle J Schalk
Jul 6, 2006·Journal of the American Chemical Society·Rebecca J AbergelKenneth N Raymond
Dec 19, 2007·Molecular Microbiology·María A LlamasWilbert Bitter
Apr 9, 2008·Journal of Bacteriology·Mark T Anderson, Sandra K Armstrong
Oct 17, 2008·The Journal of Biological Chemistry·Xuebo LiuToshihiko Osawa
Feb 14, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Silke I MüllerKlaus Hantke
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nicola C ReadingVanessa Sperandio
Aug 22, 2009·PLoS Pathogens·David T HughesVanessa Sperandio
Oct 8, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Andre M N SilvaRobert C Hider
Oct 22, 2009·Natural Product Reports·Harald Gross, Joyce E Loper
Apr 9, 2010·Natural Product Reports·Robert C Hider, Xiaole Kong
Mar 30, 1946·Lancet·E V COOPER
May 7, 2011·Microbiology·Sivan EliasEhud Banin
Oct 4, 2011·Veterinary Microbiology·Elin VerbruggheFrank Pasmans
Jan 21, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Jérémy BrandelAnne-Marie Albrecht-Gary
Aug 31, 2012·Molecular Microbiology·Matthew F TraxlerRoberto Kolter
Dec 3, 2013·Frontiers in Cellular and Infection Microbiology·Pierre Cornelis, Jozef Dingemans
Apr 1, 2014·Cell·Yasmine Belkaid, Timothy W Hand
Jun 14, 2014·FEMS Microbiology Reviews·María A LlamasIain L Lamont
Dec 17, 2014·Redox Report : Communications in Free Radical Research·Victoria MelinDavid Contreras
Mar 1, 2015·Applied and Environmental Microbiology·Justine GaletBertrand Aigle
Jul 7, 2015·Environmental Microbiology·Olivier CunrathIsabelle J Schalk
Nov 22, 2015·Advances in Experimental Medicine and Biology·Victoria V Roshchina
Oct 6, 2017·FEMS Microbiology Reviews·Sylvie ChevalierPierre Cornelis
Feb 27, 2019·Animal : an International Journal of Animal Bioscience·J M Lyte, M Lyte
Feb 26, 2020·Bioinformatics·David BouyssiéChristophe Bruley
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Quentin PerraudIsabelle J Schalk

Related Concepts

Dopamine
Environment
Epinephrine
Iron Chelating Agents
Levodopa
Norepinephrine
Pseudomonas aeruginosa
Transcription, Genetic
Catecholamine Plasma Membrane Transport Proteins
Pyochelin

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

Journal of the Royal Society, Interface
Gabriel E LeventhalKonstanze T Schiessl
Zeitschrift für die gesamte Hygiene und ihre Grenzgebiete
W MuckeS Mebel
Microbiología : Publicación De La Sociedad Española De Microbiología
M Riquelme
© 2021 Meta ULC. All rights reserved