Opposing action of estrogen receptors alpha and beta on tumor necrosis factor-alpha gene expression and caspase-8-mediated apoptotic effects in HA22T cells.

Molecular and Cellular Biochemistry
Erh-Jung HuangWei-Wen Kuo

Abstract

Hepatocellular carcinoma (HCC), the major manifestation of primary liver cancer, is one of the most frequent and malignant cancers worldwide, especially in Taiwan. Estrogen receptors (ERs) have been reported to play either a proliferation- or apoptosis-enhancing role in the differentiation of cancers, including HCC. In a previous experiment, we showed that transient overexpressed estrogen receptor-alpha induced early stage HCC cell line Hep 3B cell apoptosis by increasing the hTNF-alpha gene expression in a ligand-independent manner. To further clarify if the apoptotic effect occurs in poorly differentiated HCC cell line, HA22T, and elucidate the roles of ERs and TNF-alpha, DNA fragmentation and caspase activity were measured in late stage HCC cell line, HA22T, by measuring the expression of hER-alpha and hER-beta using a Tetracycline-inducible system (Tet-on). Increased DNA fragmentation and caspase-3 activity were found in hERbeta-overexpressed HA22T cells treated with estrogen (10(-8) M) but not in hERalpha-overexpressed HA22T cells. Using RT-PCR/PCR and western blotting in HA22T cells, overexpressed hER-beta was also found to increase the expression of hTNF-alpha mRNA and induce hTNF-alpha-dependent luciferase activity in a...Continue Reading

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Citations

Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephen J McPhersonGail P Risbridger
Oct 22, 2008·World Journal of Gastroenterology : WJG·Mamta KalraRashmi Kaul
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Apr 28, 2018·International Journal of Molecular Sciences·Bih-Cheng ChenChih-Yang Huang
Aug 3, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yun YangWen-Juan Gan
Dec 24, 2010·Journal of Agricultural and Food Chemistry·Chun-Che LinMei-Chin Yin
Feb 8, 2018·Molecular Pharmaceutics·Alessia RomaPaul A Spagnuolo

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