Opposing effects of bim and bcl-2 on lung endothelial cell migration.

American Journal of Physiology. Lung Cellular and Molecular Physiology
Cathy GrutzmacherChristine M Sorenson

Abstract

Integration of cell adhesive, survival, and proliferative processes is essential for capillary morphogenesis of endothelial cells (EC) in vitro and vascular development and function in vivo. Unfortunately, the molecular and cellular mechanisms that impact these processes are poorly defined. Here we examined how lack of bim and/or bcl-2 expression impact lung EC function. The absence of bcl-2 or bim had a significant impact on EC adhesion and migration. Lack of bcl-2 expression decreased lung EC migration, whereas lack of bim expression increased migration compared with their wild-type counterparts. Decreased adhesion to fibronectin and vitronectin was observed in both bcl-2-/- and bim-/- lung EC, with bcl-2-/- EC having very little adhesion to either matrix protein. Capillary morphogenesis was greatly diminished in bcl-2-/- EC, which correlated with decreased lung alveolarization in vivo, an angiogenesis-dependent process. We also observed aberrant production of extracellular matrix proteins, eNOS expression, and nitric oxide production in bcl-2-/- lung EC, which could contribute to inability to undergo capillary morphogenesis. The changes in cell adhesion and migration noted in the absence of bim or bcl-2 were independent of t...Continue Reading

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Citations

Oct 19, 2012·American Journal of Physiology. Renal Physiology·Cathy GrutzmacherChristine M Sorenson
Dec 16, 2011·American Journal of Physiology. Renal Physiology·Nader SheibaniChristine M Sorenson
Nov 30, 2013·International Journal of Cell Biology·Margaret E MorrisonChristine M Sorenson
Feb 19, 2015·International Journal of Molecular Sciences·Justus LieberJörg Fuchs
Feb 19, 2013·Experimental Cell Research·Fabian VogtSorin Armeanu-Ebinger
Nov 5, 2010·Developmental Biology·Shoujian WangChristine M Sorenson
Jun 25, 2017·Cellular and Molecular Life Sciences : CMLS·Emma C WatsonLeigh Coultas
May 5, 2020·PloS One·Juliana Falero-PerezChristine M Sorenson
Jul 6, 2019·Scientific Reports·Ismail S ZaitounChristine M Sorenson
Mar 11, 2020·Progress in Retinal and Eye Research·J Sebag
Nov 2, 2021·Frontiers in Cell and Developmental Biology·Christine M SorensonNader Sheibani

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