Opposing regulatory functions of positive and negative elements in UASG control transcription of the yeast GAL genes.

Molecular and Cellular Biology
R L FinleyR W West

Abstract

The yeast GAL1 and GAL10 genes are transcribed at a remarkably low basal level when galactose is unavailable and are induced by over 4 orders of magnitude when it becomes available. Approximately six negative control elements (designated GAL operators GALO1 to GALO6) are located adjacent to or overlapping four binding sites for the transcription activator GAL4 in the GAL upstream activating sequence UASG. The negative control elements contribute to the broad range of inducibility of GAL1 and GAL10 by inhibiting two GAL4/galactose-independent activating elements (GAE1 and GAE2) in UASG. In turn, multiple GAL4-binding sites in UASG are necessary for GAL4 to overcome repression by the negative control elements under fully inducing conditions. When glucose in addition to galactose is available (repressing conditions), the ability of GAL4 to activate transcription is diminished as a result of its reduced affinity for DNA and the reduced availability of inducer. Under these conditions, the negative control elements inhibit transcriptional activation from the glucose-attenuated GAL4 sites, thus accounting at least in part for glucose repression acting in cis. A normal part of transcriptional regulation of the GAL1 and GAL10 genes, the...Continue Reading

References

Jan 1, 1989·Genome Génome / Conseil National De Recherches Canada·V C BlasquezW T Garrard
Dec 5, 1989·Journal of Molecular Biology·E IzaurraldeU K Laemmli
Feb 1, 1988·Genes & Development·C D Clark-AdamsF Winston
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A G HinnebuschG R Fink
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D M RudenM Ptashne
Sep 1, 1987·Molecular and Cellular Biology·S B Selleck, J E Majors
Oct 1, 1987·Molecular and Cellular Biology·N F LueR D Kornberg
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K Arndt, G R Fink
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R J Bram, R D Kornberg
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, E Hoar
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, M Ptashne
Oct 25, 1981·Journal of Molecular Biology·T P St John, R W Davis
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L GuarenteP Gifford

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Citations

Jun 1, 1992·European Journal of Biochemistry·J M Gancedo
Jan 1, 1992·Molecular Microbiology·R J Trumbly
Jan 1, 1995·Molecular Microbiology·M KünzlerG H Braus
Apr 25, 2000·Gene·K MelcherM Nolden
Jul 1, 1994·Journal of Virology·W D Cress, J R Nevins

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