Opposing roles of zyxin/LPP ACTA repeats and the LIM domain region in cell-cell adhesion.

The Journal of Biological Chemistry
Marc D H Hansen, Mary C Beckerle

Abstract

Cadherins mediate cell-cell adhesion by linking cell junctions to actin networks. Although several actin regulatory systems have been implicated in cell-cell adhesion, it remains unclear how such systems drive cadherin-actin network formation and how they are regulated to coincide with initiation of adhesion. Previous work implicated VASP in assembly of cell-cell junctions in keratinocytes and the VASP-binding protein zyxin colocalizes with VASP at cell-cell junctions. Here we examine how domains in zyxin and its relative LPP contribute to cell-cell junction assembly. Using a quantitative assay for cell-cell adhesion, we demonstrate that zyxin and LPP function to increase the rate of early cell-cell junction assembly through the VASP-binding ActA repeat region. We also identify the LIM region of zyxin and LPP to be a regulatory domain that blocks function of these proteins. Deletion of the LIM domains drives adhesion and increases VASP level in detergent insoluble cadherin-actin. Dominant-negative zyxin/LPP mutants reduce the rate of adhesion, lower VASP levels in detergent-insoluble cadherin-actin networks, and allow for the accumulation of capping protein at cell-cell contacts. These data implicate the LIM domains of zyxin an...Continue Reading

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Citations

Aug 31, 2010·The Journal of Biological Chemistry·Thuc Nghi NguyenSoichiro Yamada
Aug 1, 2012·Journal of Signal Transduction·Cristina BertocchiRonen Zaidel-Bar
Feb 22, 2013·Molecular Biology of the Cell·Claire LecroiseyKathrin Gieseler
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Aug 25, 2009·Translational Oncology·Thomas Gp GrunewaldElke Butt
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Apr 17, 2008·Biochemical and Biophysical Research Communications·Marc D H Hansen, Mary C Beckerle
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Jul 22, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Patricia J RenfranzMary C Beckerle
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Jan 2, 2021·Frontiers in Physiology·Hannah E BoycottPaul Robinson

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