Opposite developmental regulation of P- and Q-type calcium currents during ontogenesis of large diameter mouse sensory neurons

Neuroscience
C HilaireJ Valmier

Abstract

Analysis of neuronal development has emphasized the importance of voltage-activated Ca2+ currents during the initial period of differentiation. We investigated non-N, non-L Ba2+ currents through Ca2+ channels in freshly dissociated large diameter embryonic mouse dorsal root ganglion neurons using the whole-cell patch-clamp technique. Two types of omega-agatoxin IVA-sensitive currents were clearly distinguished at embryonic day 13: a sustained P-type current blocked selectively at 30 nM (IC50 = 3nM) and an inactivating Q-type current blocked in the range 50-500 nM (IC50 = 120nM). The P-type Ca2+ current disappeared at day 15 whereas the Q-type Ca2+ current increased two- to three-fold during the same embryonic period. In contrast, the contribution of the non-L, non-N, omega-agatoxin IVA-resistant current (R-type) was constant during this developmental span. In conclusion, our results clearly show that P- and Q-type Ca2+ currents are differentially expressed during ontogenesis in large diameter dorsal root ganglion neurons. The developmental change, which occurs during the period of target innervation, could be related to specific key events such as natural neuron death and onset of synapse formation.

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Citations

Dec 14, 2002·Brain Research. Developmental Brain Research·Kathryn A Whyte, Susan A Greenfield
Jan 6, 2000·Progress in Neuro-psychopharmacology & Biological Psychiatry·L M HardingI Bermudez
Mar 15, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S ArnholdK Addicks
Sep 28, 1998·The European Journal of Neuroscience·G DesmadrylJ Valmier
Feb 19, 2002·The Journal of Physiology·Zhong-Ping FengNaweed I Syed
Mar 24, 2015·PloS One·Caroline S HirstSonja J McKeown
Oct 5, 2002·Journal of Neurophysiology·Arturas GrigaliunasCharlotte M Mistretta

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