Opposite effect of polyamines on In vitro gene expression: Enhancement at low concentrations but inhibition at high concentrations

PloS One
Ai KanemuraKenichi Yoshikawa

Abstract

Polyamines have various biological functions including marked effects on the structure and function of genomic DNA molecules. Changes in the higher-order structure of DNA caused by polyamines are expected to be closely related to genetic activity. To clarify this issue, we examined the relationship between gene expression and the higher-order structure of DNA under different polyamine concentrations. We studied the effects of polyamines, spermidine SPD(3+) and spermine SP(4+), on gene expression by a luciferase assay. The results showed that gene expression is increased by ca. 5-fold by the addition of SPD(3+) at 0.3 mM, whereas it is completely inhibited above 2 mM. Similarly, with SP(4+), gene expression is maximized at 0.08 mM and completely inhibited above 0.6 mM. We also performed atomic force microscopy (AFM) observations on DNA under different polyamine concentrations. AFM revealed that a flower-like conformation is generated at polyamine concentrations associated with maximum expression as measured by the luciferase assay. On the other hand, DNA molecules exhibit a folded compact conformation at polyamine concentrations associated with the complete inhibition of expression. Based on these results, we discuss the plausib...Continue Reading

References

May 25, 1978·Journal of Molecular Biology·L C Gosule, J A Schellman
Jan 1, 1990·Biopolymers·M O FenleyW K Olson
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Jun 1, 1996·Current Opinion in Structural Biology·V A Bloomfield
Dec 5, 1998·Seminars in Pediatric Surgery·J Z JonaS S Rothenberg
Apr 15, 1996·Physical Review Letters·K YoshikawaA R Khokhlov
May 24, 2001·Biophysical Journal·Y YamasakiK Yoshikawa
Jun 12, 2003·Journal of Molecular Biology·Sacha De CarloPatrick Schultz
Aug 29, 2003·Cellular and Molecular Life Sciences : CMLS·A C ChildsE W Gerner
Dec 16, 2011·The Journal of Chemical Physics·Yuko YoshikawaTadayuki Imanaka
Jul 15, 2015·Journal of Molecular Biology·Leonor Miller-FlemingMarkus Ralser
Jun 3, 2016·The Journal of Chemical Physics·Chika TonguKenichi Yoshikawa
Dec 25, 2016·The Journal of Chemical Physics·Akira MuramatsuKenichi Yoshikawa
Oct 29, 2017·World Journal of Microbiology & Biotechnology·Aslıhan Örs Gevrekci

❮ Previous
Next ❯

Citations

May 23, 2019·Nucleic Acids Research·Sergiy PerepelytsyaFrancesca Mocci
Jun 7, 2019·The Journal of Biological Chemistry·Pierre PlateauSylvain Blanquet
Dec 5, 2019·Organic & Biomolecular Chemistry·Mayu EsumiMakiko Tanaka
Oct 5, 2019·Scientific Reports·Manuela MinguzziRosa Maria Borzì
Sep 4, 2020·PloS One·Takashi NishioKenichi Yoshikawa
Oct 20, 2019·Scientific Reports·Takashi NishioKenichi Yoshikawa
Mar 8, 2020·Biophysics Reviews·Kanta TsumotoKenichi Yoshikawa
Feb 9, 2021·Briefings in Functional Genomics·Changlu QiLiang Cheng
Mar 4, 2021·International Journal of Molecular Sciences·Tomoki KitagawaKenichi Yoshikawa
Jun 5, 2021·Scientific Reports·Takashi NishioShin-Ichi Sato

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
Assay
AFM

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.