Opposite effects of tumour necrosis factor-alpha on type I and III collagen gene expression by human dermal fibroblasts in monolayer and three-dimensional cultures

The British Journal of Dermatology
M SatoY Miyachi

Abstract

We have recently established a novel fibroblast culture system supplemented with L-ascorbic acid 2-phosphate. The addition of L-ascorbic acid 2-phosphate enables human dermal fibroblasts to organize a three-dimensional dermis-like structure by accumulating collagens and extracellular matrices. The purpose of this study was to examine the effects of tumour necrosis factor-alpha (TNF-alpha) on collagen gene expression by human dermal fibroblasts in this culture system in comparison with monolayer culture. TNF-alpha suppressed the expression of pro alpha 1 (I) and pro alpha 1 (III) collagen mRNA in monolayer culture. In contrast, their expression was elevated in the three-dimensional culture system. TNF-alpha increased the mRNA expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 both in monolayer and three-dimensional culture. These data suggest that responses of human dermal fibroblasts to TNF-alpha are distinct under the different culture conditions. Extracellular matrices may modulate the responsiveness of fibroblasts to TNF-alpha.

References

May 31, 1990·Biochemical and Biophysical Research Communications·O IshikawaM Trojanowska
Dec 1, 1989·Archives of Surgery·H R FordR L Simmons
Mar 1, 1993·Cellular Immunology·C CastagnoliP M Richiardi

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Citations

Jan 17, 2009·Journal of Cosmetic Dermatology·Anja KnottStefan Gallinat
Nov 17, 2009·American Journal of Respiratory Cell and Molecular Biology·Cecilia M O'KaneJon S Friedland
Jun 7, 2011·Alternatives to Laboratory Animals : ATLA·Günther WeindlMonika Schäfer-Korting

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