Opposite functions of GSN and OAS2 on colorectal cancer metastasis, mediating perineural and lymphovascular invasion, respectively.

PloS One
Jin Cheon KimYong Sung Kim

Abstract

The present study aimed to identify molecules associated with lymphovascular invasion (LVI) and perineural invasion (PNI) and to examine their biological behavior in colorectal cancer (CRC). LVI- and PNI-associated molecules were identified and verified using sequential processes including (1) identification of 117 recurrence-associated genes differentially expressed on RNA-seq analysis using primary cancer tissues from 130 CRC patients with and without systemic recurrence; (2) analysis of molecules associated with LVI and PNI; (3) assessment of biological properties by measuring proliferation, anoikis, invasion/migration, epithelial-mesenchymal transition and autophagy flux; and (4) verification of disease-free survival using public datasets. Gelsolin (GSN) and 2'-5'-oligoadenylate synthetase 2 (OAS2) were associated with PNI and LVI, respectively. Invasion potential was >2-fold greater in GSN-overexpressing LoVo cells than in control cells (p<0.001-0.005), whereas OAS2-overexpressing RKO cells showed reduced invasion (p<0.001-0.005). GSN downregulated E-cadherin, β-catenin, claudin-1 and snail, and upregulated N-cadherin and ZEB1, whereas OAS2 overexpression had the opposite effects. Several autophagy-related proteins includi...Continue Reading

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Citations

Oct 4, 2019·Experimental & Molecular Medicine·Seon-Kyu KimJin Cheon Kim
Jun 18, 2020·Cancers·Marcus WurlitzerThorsten Rieckmann
Sep 21, 2019·Journal of Cellular Physiology·Jiaying WangLei Ma
Jan 20, 2021·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·April E DeveauxJennifer A Freedman
Sep 24, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Iori MotooIchiro Yasuda

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Methods Mentioned

BETA
RNA-seq
transfection
PCR
immunoprecipitation
pull down
zymography
flow cytometry

Software Mentioned

Kallisto
EdgeR
GeneMapper

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