Opposite Nuclear Dynamics of Two FRH-Dominated Frequency Proteins Orchestrate Non-Rhythmic Conidiation in Beauveria bassiana.

Cells
Sen-Miao TongMing-Guang Feng

Abstract

Non-rhythmic conidiation favors large-scale production of conidia serving as active ingredients of fungal insecticides, but its regulatory mechanism is unknown. Here, we report that two FREQUENCY (FRQ) proteins (Frq1/2) governed by a unique FRQ-interacting RNA helicase (FRH) orchestrate this valuable trait in Beauveria bassiana, an asexual insect-pathogenic fungus. Frq1 (964 aa) and Frq2 (583 aa) exhibited opposite expression dynamics (rhythms) in nucleus and steadily high expression levels in cytoplasm under light or in darkness no matter whether one of them was present or absent. Such opposite nuclear dynamics presented a total FRQ (pooled Frq1/2) level sufficient to persistently activate central developmental pathway in daytime and nighttime and supports continuous (non-rhythmic) conidiation for rapid maximization of conidial production in a fashion independent of photoperiod change. Importantly, both nuclear dynamics and cytoplasmic stability of Frq1 and Frq2 were abolished in the absence of the FRH-coding gene nonessential for the fungal viability, highlighting an indispensability of FRH for the behaviors of Frq1 and Frq2 in both nucleus and cytoplasm. These findings uncover a novel circadian system more complicated than t...Continue Reading

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Citations

May 15, 2020·Applied Microbiology and Biotechnology·Sen-Miao Tong, Ming-Guang Feng
Jun 26, 2021·Applied Microbiology and Biotechnology·Qi ZhouMing-Guang Feng

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Datasets Mentioned

BETA
AAA57121
U55763

Methods Mentioned

BETA
PCR
transgenic
confocal microscopy
Protein Assay
scanning electronic microscopy
dissection

Software Mentioned

ImageJ
DNAman

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