Opposite Polarity Monospore Genome De Novo Sequencing and Comparative Analysis Reveal the Possible Heterothallic Life Cycle of Morchella importuna

International Journal of Molecular Sciences
Wei LiuYinbing Bian

Abstract

Morchella is a popular edible fungus worldwide due to its rich nutrition and unique flavor. Many research efforts were made on the domestication and cultivation of Morchella all over the world. In recent years, the cultivation of Morchella was successfully commercialized in China. However, the biology is not well understood, which restricts the further development of the morel fungus cultivation industry. In this paper, we performed de novo sequencing and assembly of the genomes of two monospores with a different mating type (M04M24 and M04M26) isolated from the commercially cultivated strain M04. Gene annotation and comparative genome analysis were performed to study differences in CAZyme (Carbohydrate-active enzyme) enzyme content, transcription factors, duplicated sequences, structure of mating type sites, and differences at the gene and functional levels between the two monospore strains of M. importuna. Results showed that the de novo assembled haploid M04M24 and M04M26 genomes were 48.98 and 51.07 Mb, respectively. A complete fine physical map of M. importuna was obtained from genome coverage and gene completeness evaluation. A total of 10,852 and 10,902 common genes and 667 and 868 endemic genes were identified from the ...Continue Reading

References

May 30, 2002·Nucleic Acids Research·Arthur L DelcherSteven L Salzberg
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Sep 4, 2003·Genome Research·Li LiDavid S Roos
Oct 7, 2003·Annual Review of Microbiology·Marie-Josée Daboussi, Pierre Capy
Oct 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Emmanuel J P DouzeryHervé Philippe
Mar 25, 2005·The Journal of Heredity·H J Dalgleish, K M Jacobson
May 26, 2005·Mycological Research·Segula Masaphy
Dec 24, 2005·Nature·Masayuki MachidaHisashi Kikuchi
Mar 18, 2006·Bioinformatics·Tijl De BieMatthew W Hahn
Jun 14, 2006·Mycological Research·Richard S Winder
Jul 18, 2006·Combinatorial Chemistry & High Throughput Screening·Mahfuz ElmastaşHassan Y Aboul-Enein
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 19, 2007·Mycological Research·David S HibbettNing Zhang
Mar 15, 2008·Genome Research·Jonathan ButlerDavid B Jaffe
May 27, 2008·Fungal Genetics and Biology : FG & B·Guus BakkerenJan Schirawski
Jul 26, 2008·Fungal Genetics and Biology : FG & B·Jorge H Ramirez-PradoIgnazio Carbone
Sep 16, 2008·FEMS Microbiology Letters·Ekaterina Shelest
Sep 17, 2008·Current Protocols in Bioinformatics·Julie D ThompsonDes G Higgins
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Jun 29, 2010·Fungal Genetics and Biology : FG & B·Hatira TaşkinKerry O'Donnell
Dec 15, 2010·Bioinformatics·Marten BoetzerWalter Pirovano
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
Jan 6, 2012·Nucleic Acids Research·Yupeng WangAndrew H Paterson
Apr 17, 2012·Fungal Genetics and Biology : FG & B·Xi-Hui DuZhu L Yang
May 31, 2012·Nucleic Acids Research·Yanbin YinYing Xu
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg

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Citations

Jan 17, 2020·International Journal of Molecular Sciences·Wei LiuYinbing Bian
Sep 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qianqian ZhangYinbing Bian
Jul 29, 2021·Microbiology and Molecular Biology Reviews : MMBR·Xi-Hui Du, Zhu L Yang

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Datasets Mentioned

BETA
GME3124
SRR7448150-SRR7448158

Methods Mentioned

BETA
Illumina sequencing

Software Mentioned

Hisat2
Benchmarking Universal Single - Copy Orthologs ( BUSCO )
Trimmomatic
Pilon
finisherSC
RepeatMasker
BLASTP
r8s
GATK
Blast2go

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