PMID: 8599610Feb 29, 1996Paper

Opposite regulation of IGF-I and IGF-I receptor mRNA and concomitant changes of GH receptor and IGF-II/M6P receptor mRNA in human IM-9 lymphoblasts

Biochimica Et Biophysica Acta
Y YangW Kiess

Abstract

Human IM-9 lymphoblasts synthesize IGF-I and express IGF-I receptors, IGF-II/M6P receptors and GH receptors. We have studied the regulation of mRNA expression of IGF-I, IGF-I receptors, IGF-II/M6P receptors and GH receptors in IM-9 cells upon serum-withdrawal and re-addition of serum. IM-9 cells were cultured in RPMI-1640 medium with or without serum for various periods of time. RNA was prepared using guanidinium thiocyanate and CsCl. Antisense riboprobes for human IGF-I, IGF-I receptor, IGF-II/M6P receptor, GH receptor and for comparison for human beta-actin were synthesized and labeled with 32P. Protected fragments of 379 bases and of 420 and 350 bases with the IGF-I receptor and with the IGF-I probe respectively and protected fragments of 670 bases and of 51 and 121 bases with the GH receptor and with the beta-actin probe were detected. For the human IGF-II/M6P receptor probe protected fragments of 260 bases were visualized in RNA samples. The amount of mRNA present in each lane (10 microgram total RNA) was determined by computed densitometry. The amount of IGF-mRNA expressed by IM-9 cells decreased rapidly (within two hours) and dramatically (more than 120%) after the withdrawal of serum and increased significantly (220%) a...Continue Reading

References

Jan 1, 1991·Growth Factors·P Nissley, W Lopaczynski
Apr 30, 1990·Biochemical and Biophysical Research Communications·N HattoriH Imura
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WernerD LeRoith
Mar 1, 1989·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·G A WertherR G Rosenfeld
Nov 1, 1989·The Journal of Clinical Endocrinology and Metabolism·T J MerimeeL L Cavalli-Sforza
Mar 1, 1994·Molecular and Cellular Endocrinology·Z HanJ H Wyche

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Citations

Apr 14, 2015·BioMed Research International·Varsha P BrahmkhatriHanudatta S Atreya
Sep 19, 2007·American Journal of Clinical Pathology·Jitakshi DeBruce H Davis

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