Opsin/all-trans-retinal complex activates transducin by different mechanisms than photolyzed rhodopsin

Biochemistry
S JägerK P Hofmann

Abstract

In rhodopsin, the 11-cis-retinal chromophore forms a complex with Lys296 of opsin via a protonated Schiff base. Absorption of light initiates the activation of rhodopsin by cis/trans photoisomerization of retinal. Thermal relaxation through different intermediates leads into the metarhodopsin states which bind and activate transducin (Gt) and rhodopsin kinase (RK). all-trans-Retinal also recombines with opsin independent of light, forming activating species of the receptor. In this study, we examined the mechanism by which all-trans-retinal activates opsin. To exclude other amines except active site Lys296 from formation of Schiff bases, we reductively methylated rhodopsin (PM-rhodopsin), which we then bleached to generate PM-opsin. Using spectroscopic methods and a Gt activation assay, we found that all-trans-retinal interacted with PM-opsin, producing a noncovalent complex that activated Gt. The residual nucleotide exchange in Gt catalyzed by opsin was approximately 1/250 lower relative to that of photoactivated rhodopsin (pH 8.0, 23 degrees C). Addition of equimolar all-trans-retinal led to an occupancy of one-tenth of the putative retinal binding site(s) of opsin and enhanced the Gt activation rate 2-fold. When the concentr...Continue Reading

References

Apr 1, 1978·The Journal of General Physiology·D R PepperbergJ E Dowling
Apr 1, 1992·Journal of Bioenergetics and Biomembranes·J W Lewis, D S Kliger
Mar 1, 1988·Biophysical Journal·R R BirgeL P Murray
Dec 1, 1980·The Journal of Physiology·D A BaylorK W Yau
Jul 1, 1982·The Journal of Physiology·D A Baylor, T D Lamb
Jun 16, 1995·The Journal of Biological Chemistry·H OhguroK Palczewski
Mar 10, 1995·The Journal of Biological Chemistry·A SuryaB E Knox

❮ Previous
Next ❯

Citations

Oct 29, 1996·Biochimica Et Biophysica Acta·E J Helmreich, K P Hofmann
Aug 1, 1997·Current Opinion in Neurobiology·K Palczewski, J C Saari
Nov 9, 2000·Progress in Retinal and Eye Research·T Ebrey, Y Koutalos
Jun 8, 2001·Progress in Retinal and Eye Research·J K McBeeD R Pepperberg
Oct 6, 2001·Progress in Retinal and Eye Research·I M Pepe
Jun 9, 2009·Visual Neuroscience·Tomoki IsayamaC L Makino
Dec 24, 2011·Visual Neuroscience·Sadaharu MiyazonoClint L Makino
Mar 8, 2011·Biochemistry·Ali I KayaHeidi E Hamm
Oct 17, 1998·Eye·C S LeibrockT D Lamb
Aug 11, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Chunhe Chen, Yiannis Koutalos
Dec 3, 1999·European Journal of Biochemistry·C SeibertK P Hofmann
May 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas E AngelKrzysztof Palczewski
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Takahiro YamashitaYoshinori Shichida
Jan 8, 2010·The Journal of Biological Chemistry·Hisao TsukamotoYoshinori Shichida
Nov 13, 2002·The Journal of Biological Chemistry·Martin HeckKlaus Peter Hofmann
Apr 23, 2003·The Journal of Biological Chemistry·Sandra A SchadelKlaus Peter Hofmann
Dec 20, 2003·The Journal of Biological Chemistry·Matthew L BattenKrzysztof Palczewski
Mar 10, 2005·The Journal of Biological Chemistry·Akiko MaedaKrzysztof Palczewski
Mar 24, 2009·The Journal of Biological Chemistry·Akiko MaedaKrzysztof Palczewski
Jul 19, 2006·The Journal of General Physiology·Petri Ala-LaurilaM Carter Cornwall
Nov 15, 2006·The Journal of General Physiology·Maureen E EstevezM Carter Cornwall
Jul 29, 2009·The Journal of General Physiology·Maureen E EstevezM Carter Cornwall
Aug 19, 2009·The Journal of General Physiology·Kiyoharu J MiyagishimaAlapakkam P Sampath
Oct 2, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Beata JastrzebskaKrzysztof Palczewski
Sep 2, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yoshinori Shichida, Take Matsuyama
Oct 1, 1996·Photochemistry and Photobiology·R K CrouchD R Pepperberg
Feb 5, 2009·Photochemistry and Photobiology·David T LodowskiKrzysztof Palczewski
May 21, 2013·Annual Review of Neuroscience·Krzysztof Palczewski, Tivadar Orban
Jun 8, 2006·Annual Review of Biochemistry·Krzysztof Palczewski
Sep 14, 2006·Annual Review of Pharmacology and Toxicology·Gabriel H TravisKrzysztof Palczewski
Feb 12, 2008·Investigative Ophthalmology & Visual Science·Ambarish S PawarDavid R Pepperberg
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joseph A GoncalvesSteven O Smith
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Akiko MaedaKrzysztof Palczewski
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·O G KisselevK P Hofmann
Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hisao TsukamotoYoshinori Shichida
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·A V CideciyanK Palczewski
Feb 11, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Andreas OckenfelsWolfgang Gärtner
Jul 5, 2005·The Journal of Physiology·Jie FanGordon L Fain
Jun 9, 2005·Experimental Eye Research·Young Hee KimGyeong Jae Cho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.