Opsonic requirements of Helicobacter pylori

Journal of Medical Microbiology
A W McKinlayC G Gemmell

Abstract

The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the bacteria with NHS 30% caused the release of radiolabel and this effect was abolished by heating serum to 56 degrees C, suggesting that H. pylori is serum-sensitive and that complement is involved. Opsonisation of H. pylori with NHS concentrations of 10-30% significantly increased PMNL chemiluminescence. Removal of specific antibody had no effect. Removal of either the classical or alternative complement pathways produced no significant change in PMNL chemiluminescence, indicating that either pathway is sufficient for opsonisation on its own. The results confirm that complement is the most efficient opsonin for H. pylori.

Citations

Jan 1, 1994·Journal of Gastroenterology and Hepatology·C S Goodwin
Mar 27, 2001·Gastroenterology·A E BerstadP Brandtzaeg
Jan 1, 1996·Scandinavian Journal of Gastroenterology. Supplement·R A VeenendaalC B Lamers
Mar 1, 1996·Helicobacter·G Gonzalez-ValenciaM J Blaser
Jan 24, 2019·Plant Disease·Japheth D Weems, Carl A Bradley

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