PMID: 6103873Mar 1, 1980

Opsonization of four Bacteroides species: role of the classical complement pathway and immunoglobulin

Infection and Immunity
R W TofteP G Quie

Abstract

Previous investigators have suggested that opsonization of two Bacteroides species is mediated exclusively by the alternative complement pathway and requires immunoglobulins. In this study, the nature of the opsonic factors in nonimmune human serum for four species of Bacteroides was investigated by measuring uptake of [(3)H]thymidine-labeled bacteria by human polymorphonuclear leukocytes. Normal human serum, C2-deficient serum, immunoglobulin-deficient serum, and serum chelated with ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid (EGTA), MgEGTA, and ethylenediaminetetraacetic acid (EDTA) were used as opsonic sources. Heat inactivation of each of these sera significantly reduced its opsonic activity for all four Bacteroides species, suggesting that serum complement was essential for effective opsonization. All strains were opsonized in the absence of the classical complement pathway; however, kinetics studies revealed that opsonization proceeded at a significantly faster rate when the classical complement pathway was intact. Although two strains were opsonized in immunoglobulin-deficient sera, opsonization was less efficient and appeared to occur via the alternative complement pathway. Unexpectedly, all strains ...Continue Reading

Citations

Jan 1, 1987·Archives of Gynecology and Obstetrics·Itzhak Brook
Feb 28, 2008·Veterinary Ophthalmology·Eric C Ledbetter, Janet M Scarlett
Jan 1, 1985·Journal of Periodontal Research·T J van SteenbergenJ de Graaff
Sep 1, 1992·Clinical and Experimental Immunology·S GalandiukH C Polk
Oct 1, 1990·Hospital Practice·Itzhak Brook
Jan 1, 1987·Critical Reviews in Microbiology·Itzhak Brook
Jan 1, 1984·Antonie van Leeuwenhoek·D M MacLarenJ A Kaan
Jul 1, 1996·Pediatric Dermatology·Itzhak Brook
Jan 1, 1994·FEMS Microbiology Reviews·Itzhak Brook
Mar 1, 1988·Journal of Clinical Periodontology·A J van WinkelhoffJ de Graaff
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·B E ClaessonD E Holmlund
Apr 24, 2003·Pediatrics International : Official Journal of the Japan Pediatric Society·Itzhak Brook

Related Concepts

Bacteroides
Bacteroides fragilis
Prevotella melaninogenica
Complement Activation
Complement Pathway, Alternative
Complement Pathway, Classical
Edetic Acid, Sodium Salt
Alkalescens-Dispar Group
Immunoglobulins
Opsonin

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