PMID: 870027May 3, 1977Paper

Optical activity and conformation of cobra neurotoxin

Biochemistry
Y H ChenJ T Yang

Abstract

Cobra neurotoxin from Formosan cobra (Naja naja atra) venom is a compact globular protein having an intrinsic viscosity of 4.5 mL/g. The protein is stable in 7.5 M urea but can be denatured in 4.1 M guanidine hydrochloride or at elevated temperature (above 70 degrees C). Its conformation remains virtually the same in solvents of lower polarity than water such as 1,2-ethanediol or a mixed solvent of 1-propanol-1,2-ethanediol-water (5:1:1 by volume). The circular dichroism spectrum is "atypical" in water in that the peptide chromophores show a small negative circular dichroic (CD) band at 215 nm, a large positive one at 199 nm, and another large negative one below 190 nm. The CD pattern resembles to some extent that of a beta form but differs in both positions and magnitudes from the latter. It agrees qualitatively with the theoretical calculations of the reverse beta bends, suggesting that cobra toxin contains a considerable amount of beta turns and possibly a mixture of beta form and beta turns.

Citations

Aug 21, 1978·Biochimica Et Biophysica Acta·M C HungY H Chen
Dec 20, 1978·Biochimica Et Biophysica Acta·T H HseuC C Yang
Jan 1, 1983·The International Journal of Biochemistry·A Gałat
Apr 28, 1981·Biochimica Et Biophysica Acta·J G GavilanesM Oñaderra
Jan 1, 1980·CRC Critical Reviews in Biochemistry·J A Smith, L G Pease
Jan 1, 1983·CRC Critical Reviews in Biochemistry·M J Dufton, R C Hider
Mar 28, 1978·Biochimica Et Biophysica Acta·L Visser, A I Louw
Nov 1, 1978·Analytical Biochemistry·C T ChangJ T Yang
Sep 2, 1980·Biochemistry·A MenezP Fromageot
Dec 22, 1981·Biochemistry·A GałatE R Blout
Oct 1, 1977·International Journal of Peptide and Protein Research·M C Hung, Y H Chen
Mar 1, 1978·International Journal of Peptide and Protein Research·K NaritaT B Lo
Mar 1, 1980·International Journal of Peptide and Protein Research·Y BirkT A Bewley
Nov 1, 1978·International Journal of Peptide and Protein Research·T A Bewley, Y Birk

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1-Propanol
Circular Dichroism, Vibrational
Ethylene Glycols
Guanidines
Optical Rotation
Protein Conformation
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Carmol

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