Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence

Journal of Biomedical Optics
Alain BouchardAkier Assanta Mafu

Abstract

A scanning optical system for the detection of bacteria on meat surfaces based on fluorescence lifetime and intensity measurements is described. The system detects autofluorescent light emitted by naturally occurring fluorophores in bacteria. The technique only requires minimal sample preparation and handling, thus the chemical properties of the specimen are preserved. This work presents the preliminary results obtained from a time-resolved fluorescence imaging system for the characterization of a nonpathogenic gram-negative bacteria, Pseudomonas fluorescens. Initial results indicate that the combination of fluorescence lifetime and intensity measurements provides a means for characterizing biological media and for detecting microorganisms on surfaces.

References

Jul 14, 2001·Fresenius' Journal of Analytical Chemistry·M Ihnat
Dec 12, 2002·Biochemistry·Lisa M Gloss, Brandon J Placek
Jun 1, 1991·Journal of Fluorescence·J R LakowiczD Gloyna

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