Optical coherence tomography visualizes neurons in human entorhinal cortex

Neurophotonics
Caroline MagnainBruce Fischl

Abstract

The cytoarchitecture of the human brain is of great interest in diverse fields: neuroanatomy, neurology, neuroscience, and neuropathology. Traditional histology is a method that has been historically used to assess cell and fiber content in the ex vivo human brain. However, this technique suffers from significant distortions. We used a previously demonstrated optical coherence microscopy technique to image individual neurons in several square millimeters of en-face tissue blocks from layer II of the human entorhinal cortex, over 50 µm in depth. The same slices were then sectioned and stained for Nissl substance. We registered the optical coherence tomography (OCT) images with the corresponding Nissl stained slices using a nonlinear transformation. The neurons were then segmented in both images and we quantified the overlap. We show that OCT images contain information about neurons that is comparable to what can be obtained from Nissl staining, and thus can be used to assess the cytoarchitecture of the ex vivo human brain with minimal distortion. With the future integration of a vibratome into the OCT imaging rig, this technique can be scaled up to obtain undistorted volumetric data of centimeter cube tissue blocks in the near t...Continue Reading

Citations

Dec 22, 2015·Neuron·Katrin Amunts, Karl Zilles
Oct 1, 2015·Optics Letters·Eunjung MinWoonggyu Jung
Apr 6, 2016·Frontiers in Neuroanatomy·Stephen P AmatoTimothy M Ragan
Oct 16, 2015·Nature·Russell A Poldrack, Martha J Farah
Apr 30, 2016·Optics Letters·Caroline MagnainDavid A Boas
May 14, 2016·Optics Letters·Hui WangDavid Boas
Oct 11, 2016·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Jing MenChao Zhou
Jul 16, 2016·The Journal of Comparative Neurology·Song-Lin DingEd S Lein
Dec 23, 2016·Scientific Reports·Eunjung MinCatherine Best-Popescu
Dec 8, 2017·Journal of Medical Engineering·Mobin Ibne Mokbul
Jun 5, 2018·NMR in Biomedicine·Alard RoebroeckManisha Aggarwal
Oct 12, 2016·Neurophotonics·Chao J LiuTaner Akkin
Sep 1, 2017·NMR in Biomedicine·Svenja Caspers, Markus Axer
Jul 19, 2017·Biomedical Optics Express·Paul J MarchandTheo Lasser

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