Optical dissection of stimulus-evoked retinal activation

Optics Express
Xin-Cheng Yao, You-Bo Zhao

Abstract

Better understanding of stimulus-evoked intrinsic optical signals (IOSs) in the retina promises new methodology for study and diagnosis of retinal function. Using a flood-illumination near infrared (NIR) light microscope equipped with high-speed CCD (80 Hz) and CMOS (1000 Hz) cameras, we validated depth-resolved enface imaging of fast IOSs in isolated retina of leopard frog. Both positive (increasing) and negative (decreasing) IOSs were observed at the photoreceptor and inner layers of the retina. The distribution of IOSs with opposite polarities showed a center-surround pattern. At the photoreceptor layer, negative IOSs dominated the center area illuminated by the stimulus light spot, while positive signals dominated the surrounding area. In contrast, at inner retinal layers, positive IOSs dominated the center area covered by the stimulus light spot, and negative IOSs were mainly observed in the surrounding area. Fast CMOS imaging disclosed rapid IOSs within 5 ms after the stimulus onset, and both ON and OFF optical responses were observed associated with a step light stimulus.

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Citations

Sep 19, 2009·Japanese Journal of Ophthalmology·Kazushige TsunodaManabu Tanifuji
Sep 19, 2009·Japanese Journal of Ophthalmology·Xin-Cheng Yao
Jun 24, 2016·Journal of Biomedical Optics·Xiaohui ZhaoXincheng Yao
Sep 23, 2016·Journal of Biomedical Optics·Benquan WangXincheng Yao
Mar 29, 2012·Optics Express·Qiu-Xiang ZhangXin-Cheng Yao
Jun 20, 2020·Experimental Biology and Medicine·Xincheng Yao, Tae-Hoon Kim
Nov 18, 2010·Optics Letters·Qiu-Xiang ZhangXin-Cheng Yao
Nov 30, 2018·Experimental Biology and Medicine·Xincheng YaoYiming Lu
Sep 21, 2013·Biomedical Optics Express·Rong-Wen LuXin-Cheng Yao
Jun 24, 2011·Biomedical Optics Express·Rong-Wen LuXin-Cheng Yao

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