PMID: 6991590May 1, 1980Paper

Optical errors in scanning stage absorbance cytophotometry. II. Application of correction factors for residual distributional error, glare and diffraction error in practical cytophotometry

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
W A DuijndamS H Riddersma

Abstract

In a preceding article theoretical methods were derived for correcting the integrated absorbance values of microscopic objects determined with a scanning stage cytophotometer for the systematical erros due to residual distributional error, diffraction error, and glare error. For an experimental investigation of these results, the local apparant transmission at the center of an opaque particle was determined and this value used as a measure for the substage glare. By sufficient reduction of the size of the illuminated field, this substage glare could be kept below 1% in our scanning stage cytophotometer. The magnitude of the diffraction error was experimentally approached by comparing the values found for the integrated absorbance of the same amount of chromophore, dispersed over two different areas by crushing or centrifugation. After appropriate correction for the residual distributional error, the remaining difference was ascribed to the diffraction error, caused by diffraction at the edges of the object, and to the glare error, present all over the measured area. The local borderline corrections found necessary to obtain the best matching corrected integrated absorbance values were between 3 and 5%, in good agreement with th...Continue Reading

Citations

May 1, 1983·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E JacobsenE W Bijmolt
Mar 1, 1981·The Histochemical Journal·D Glick
Mar 1, 1981·The Histochemical Journal·D J Goldstein
Apr 25, 2012·Molecular Neurobiology·Thomas Arendt
Jan 1, 1982·Histochemistry·E ProsperiM van der Ploeg
Apr 19, 2012·Aging Cell·Hans-Georg FischerThomas Arendt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.