Optical fiber-based in situ spectroscopy of pigmented single colonies.

Applied and Environmental Microbiology
M WiggliR Bachofen

Abstract

We have adapted a commercially available fiber-optic spectroradiometer with diode array detection to record reflection and absorption spectra from single, 1-mm-diameter bacterial colonies. A careful assessment of the performance of the spectroradiometer for this application is reported. In a model study employing colonies from various phototrophic bacteria, we show that the reflectance spectra are reliable within the range of 450 to 820 nm, whereas the transmission spectra yield accurate peak intensities and absorption maxima from 400 to 900 nm. For screening of populations of about 10(sup4) colonies, fiber-optic transmission spectroscopy provides an attractive and inexpensive alternative to present techniques based on charge-coupled device imaging technology.

References

May 9, 1979·Biochimica Et Biophysica Acta·M Snozzi, R Bachofen
May 5, 1994·Nature·D C Youvan
Feb 11, 1994·Science·M ChalfieD C Prasher
Oct 1, 1959·Archives of Biochemistry and Biophysics·P LATIMER, E RABINOWITCH
Jun 1, 1960·Journal of General Microbiology·W R SISTROM
Aug 1, 1962·Archives of Biochemistry and Biophysics·P LATIMER, C A EUBANKS
Dec 1, 1993·Applied and Environmental Microbiology·D LestanA Perdih

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Citations

Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Byron F Brehm-Stecher, Eric A Johnson

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