Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy

Light, Science & Applications
Murad OmarVasilis Ntziachristos

Abstract

Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.

References

May 10, 2008·Journal of Biomedical Optics·John GamelinQuing Zhu
Aug 3, 2010·Nature Methods·Vasilis Ntziachristos
Apr 10, 1994·Applied Optics·L P Yaroslavsky, H J Caulfield
Jul 10, 2012·Nature Methods·Pierre MahouEmmanuel Beaurepaire
Aug 7, 2012·Interface Focus·Paul Beard
Aug 14, 2013·Optics Letters·Murad OmarVasilis Ntziachristos
Oct 8, 2013·Journal of Biomedical Optics·Jérôme GateauVasilis Ntziachristos
Apr 2, 2014·Optics Letters·George J TserevelakisVasilis Ntziachristos
Jul 1, 2014·Optics Letters·Stratis TzoumasVasilis Ntziachristos
Jul 1, 2014·Optics Letters·Murad OmarVasilis Ntziachristos
Jan 6, 2015·Optica·Liren ZhuLihong V Wang
Mar 10, 2015·Neoplasia : an International Journal for Oncology Research·Murad OmarVasilis Ntziachristos
Aug 27, 2015·Scientific Reports·Dominik SolimanVasilis Ntziachristos
Sep 30, 2015·Biomedical Optics Express·Andrei ChekkouryVasilis Ntziachristos
May 29, 2016·Developmental Biology·Maria Angels de Luis BalaguerRosangela Sozzani

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Citations

Feb 1, 2019·Journal of Biophotonics·Benedict Mc LarneyDaniel Razansky
Mar 14, 2019·Journal of Biophotonics·Renzhe BiMalini Olivo
Apr 17, 2019·Nature Biomedical Engineering·Murad OmarVasilis Ntziachristos
Apr 29, 2020·Biomedical Optics Express·Kalloor Joseph FrancisWiendelt Steenbergen
Mar 14, 2020·Nature Biomedical Engineering·Katja HaedickeJan Grimm
Mar 10, 2017·Chemical Society Reviews·X L Deán-BenD Razansky
Dec 29, 2020·Physics in Medicine and Biology·Dhiman DasManojit Pramanik
Jan 22, 2019·ACS Sensors·Antonella LauriGil G Westmeyer

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Methods Mentioned

BETA
X-ray
optical diffraction

Software Mentioned

RSOM
MORSOM
SPIM

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