Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress.

Journal of Biomedical Optics
Reyhaneh SepehrMahsa Ranji

Abstract

Ventilation with enhanced fractions of O(2) (hyperoxia) is a common and necessary treatment for hypoxemia in patients with lung failure, but prolonged exposure to hyperoxia causes lung injury. Ischemia-reperfusion (IR) injury of lung tissue is common in lung transplant or crush injury to the chest. These conditions are associated with apoptosis and decreased survival of lung tissue. The objective of this work is to use cryoimaging to evaluate the effect of exposure to hyperoxia and IR injury on lung tissue mitochondrial redox state in rats. The autofluorescent mitochondrial metabolic coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are electron carriers in ATP generation. These intrinsic fluorophores were imaged for rat lungs using low-temperature fluorescence imaging (cryoimaging). Perfused lungs from four groups of rats were studied: normoxia (control), control perfused with an mitochondrial complex IV inhibitor (potassium cyanide, KCN), rats exposed to hyperoxia (85% O(2)) for seven days, and from rats subjected to lung IR in vivo 24 hours prior to study. Each lung was sectioned sequentially in the transverse direction, and the images were used to reconstruct a three-dimensional (3-D) ...Continue Reading

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Citations

Jan 12, 2013·European Journal of Applied Physiology·Huai-Dong LiJin-Song Chen
Dec 15, 2012·Annals of Biomedical Engineering·Kevin StaniszewskiMahsa Ranji
Jan 8, 2013·Journal of Biomedical Optics·Sepideh MalekiMahsa Ranji
Mar 29, 2014·Journal of Innovative Optical Health Sciences·Reyhaneh SepehrMahsa Ranji
Jun 12, 2014·Journal of Innovative Optical Health Sciences·He N XuLin Z Li
Aug 9, 2017·Pflügers Archiv : European journal of physiology·Baher A IbrahimDaniel A Llano
Jan 21, 2018·Journal of Biomedical Optics·Mette Funding la CourAmadou K S Camara
Oct 30, 2016·Journal of Biomedical Optics·Silas J LeavesleyCarole W Boudreaux
Feb 9, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Cui MaElizabeth R Jacobs
Nov 30, 2016·Biomedical Optics Express·Kinan AlhallakNarasimhan Rajaram
Dec 5, 2013·Experimental Biology and Medicine·Zahra GhanianMahsa Ranji
Sep 25, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Andreas M BeyerElizabeth R Jacobs

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