Optical investigation of osteoarthritic human cartilage (ICRS grade) by confocal Raman spectroscopy: a pilot study

Analytical and Bioanalytical Chemistry
Rajesh KumarMagnus B Lilledahl

Abstract

Biomolecular changes in the cartilage matrix during the early stage of osteoarthritis may be detected by Raman spectroscopy. The objective of this investigation was to determine vibrational spectral differences among different grades (grades I, II, and III) of osteoarthritis in human osteoarthritic cartilage, which was classified according to the International Cartilage Repair Society (ICRS) grading system. Degenerative articular cartilage samples were collected during total joint replacement surgery and were classified according to the ICRS grading system for osteoarthritis. Twelve cartilage sections (4 sections of each ICRS grades I, II, and III) were selected for Raman spectroscopic analysis. Safranin-O/Fast green was used for histological staining and assignment of the Osteoarthritis Research Society International (OARSI) grade. Multivariate principal component analysis (PCA) was used for data analysis. Spectral analysis indicates that the content of disordered coil collagen increases significantly during the early progression of osteoarthritis. However, the increase was not statistically significant during later stages of the disease. A decrease in the content of proteoglycan was observed only during advanced stages of ost...Continue Reading

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Citations

Feb 1, 2018·International Journal of Molecular Sciences·Rajesh KumarMagnus B Lilledahl
Jun 17, 2020·Expert Review of Molecular Diagnostics·Paula Casal-BeiroaJoana Magalhães
Nov 19, 2019·Frontiers in Bioengineering and Biotechnology·Mads S BergholtMichael B Albro
Jan 23, 2019·Lasers in Medical Science·Corina D HosuDaniela Fodor
Jun 3, 2021·International Journal of Molecular Sciences·Chen YuLian Li
Aug 10, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kimberly R KroupaMichael B Albro

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