Optical method for monitoring the concentration of general anesthetics and other small organic molecules. An example of phase transition sensing

Analytical Chemistry
S Merlo, P Yager

Abstract

As an example of chemical sensing based on perturbations of thermal phase transitions, we have shown that phospholipids labeled with a fluorescent dye may be used to measure the concentration of general anesthetics and other small organic molecules. The emission maximum of the hydrophobic fluorescent probe Laurdan in phospholipid bilayers shifts from a wavelength of 445 nm below the main phase transition of the lipid to 480 nm above it, with an isosbestic point at approximately 475 nm. The greatest changes in intensity at the transition occur at 440 and 500 nm, so the ratio of the intensities at these two points was used as an "order parameter". The effects of variation of the liposomal preparation method on the order parameter were explored, and it was found that in mixed lipids the parameter varied nearly linearly over the physiological temperature range. Fluorometry detected changes in the order of bilayers caused by solubilization of the anesthetic isoflurane (Forane) and of ethanol. At a defined temperature, the intensity ratio measured in the presence of anesthetic decreases in a concentration-dependent manner. Immobilizing the liposomes in a hydrogel did not perturb the response of the system. This work demonstrates the ...Continue Reading

References

Oct 19, 1979·Biochimica Et Biophysica Acta·F OlsonD Papahadjopoulos
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J R TrudellE N Cohen
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D B MountcastleM J Halsey
Jan 19, 1978·Biochimica Et Biophysica Acta·P W van DijckJ de Gier
Jan 4, 1977·Biochimica Et Biophysica Acta·J M VanderkooiG G McDonald
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Mabrey, J M Sturtevant
Jun 1, 1982·Journal of Neurochemistry·C Sumbilla, J R Lakowicz
Aug 19, 1980·Biochemistry·S E SchulleryT E Thompson
Feb 8, 1982·Biochimica Et Biophysica Acta·B P Gaber, J P Sheridan
Nov 1, 1981·Archives of Ophthalmology·D Miller, R Miller

❮ Previous
Next ❯

Citations

Jul 1, 1993·Journal of Biomedical Materials Research·B D Ratner
Jul 4, 1993·Biochimica Et Biophysica Acta·P L Chong, P T Wong
Apr 1, 1996·Biomaterials·B RangarajanA B Scranton
Sep 1, 1995·Photochemistry and Photobiology·C F ChapmanB J Tromberg
Jul 20, 2002·Photochemistry and Photobiology·Sharon VanounouItzhak Fishov
Jan 19, 2020·Journal of Fluorescence·Radha RanganathanMiroslav Peric
Apr 5, 2020·Biophysical Journal·Radha RanganathanMiroslav Peric

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.